The effect of hormones on the enzymes responsible for the synthesis (fructose-6-P,2-kinase) and degradation (fructose-2,6-Pase) of fructose-2,6-P2 was examined in isolated rat hepatocytes. Glucagon (10-11 M), epinephrine (10-5 M), or calcium (2.4 mM) and A23187 (10-5 M) administration to hepatocytes produced simultaneous activation of fructose-2,6-Pase and inactivation of fructose-6-P,2-kinase within 2 minutes. The effect of epinephrine on these two enzymes was dependent on the presence of Ca++. These results suggest that the level of fructose-2,6-P2 is controlled by recriprocal changes in fructose-2,6-Pase and fructose-6-P,2-kinase activities.
|Original language||English (US)|
|Number of pages||7|
|Journal||Biochemical and Biophysical Research Communications|
|State||Published - Feb 11 1982|
ASJC Scopus subject areas
- Molecular Biology
- Cell Biology