Reciprocal changes in fructose-6-phosphate,2-kinase and fructose-2,6-bisphosphatase activity in response to glucagon and epinephrine

Carolyn Sue Richards; Motoko Yokoyama; Eisuke Furuya; Kosaku Uyeda

doi:10.1016/0006-291X(82)91359-6

Reciprocal changes in fructose-6-phosphate,2-kinase and fructose-2,6-bisphosphatase activity in response to glucagon and epinephrine

Carolyn Sue Richards, Motoko Yokoyama, Eisuke Furuya, Kosaku Uyeda

Research output: Contribution to journal › Article › peer-review

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Abstract

The effect of hormones on the enzymes responsible for the synthesis (fructose-6-P,2-kinase) and degradation (fructose-2,6-Pase) of fructose-2,6-P₂ was examined in isolated rat hepatocytes. Glucagon (10^-11 M), epinephrine (10^-5 M), or calcium (2.4 mM) and A23187 (10^-5 M) administration to hepatocytes produced simultaneous activation of fructose-2,6-Pase and inactivation of fructose-6-P,2-kinase within 2 minutes. The effect of epinephrine on these two enzymes was dependent on the presence of Ca⁺⁺. These results suggest that the level of fructose-2,6-P₂ is controlled by recriprocal changes in fructose-2,6-Pase and fructose-6-P,2-kinase activities.

Original language	English (US)
Pages (from-to)	1073-1079
Number of pages	7
Journal	Biochemical and Biophysical Research Communications
Volume	104
Issue number	3
DOIs	https://doi.org/10.1016/0006-291X(82)91359-6
State	Published - Feb 11 1982

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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title = "Reciprocal changes in fructose-6-phosphate,2-kinase and fructose-2,6-bisphosphatase activity in response to glucagon and epinephrine",

abstract = "The effect of hormones on the enzymes responsible for the synthesis (fructose-6-P,2-kinase) and degradation (fructose-2,6-Pase) of fructose-2,6-P2 was examined in isolated rat hepatocytes. Glucagon (10-11 M), epinephrine (10-5 M), or calcium (2.4 mM) and A23187 (10-5 M) administration to hepatocytes produced simultaneous activation of fructose-2,6-Pase and inactivation of fructose-6-P,2-kinase within 2 minutes. The effect of epinephrine on these two enzymes was dependent on the presence of Ca++. These results suggest that the level of fructose-2,6-P2 is controlled by recriprocal changes in fructose-2,6-Pase and fructose-6-P,2-kinase activities.",

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AU - Yokoyama, Motoko

AU - Furuya, Eisuke

AU - Uyeda, Kosaku

PY - 1982/2/11

Y1 - 1982/2/11

N2 - The effect of hormones on the enzymes responsible for the synthesis (fructose-6-P,2-kinase) and degradation (fructose-2,6-Pase) of fructose-2,6-P2 was examined in isolated rat hepatocytes. Glucagon (10-11 M), epinephrine (10-5 M), or calcium (2.4 mM) and A23187 (10-5 M) administration to hepatocytes produced simultaneous activation of fructose-2,6-Pase and inactivation of fructose-6-P,2-kinase within 2 minutes. The effect of epinephrine on these two enzymes was dependent on the presence of Ca++. These results suggest that the level of fructose-2,6-P2 is controlled by recriprocal changes in fructose-2,6-Pase and fructose-6-P,2-kinase activities.

AB - The effect of hormones on the enzymes responsible for the synthesis (fructose-6-P,2-kinase) and degradation (fructose-2,6-Pase) of fructose-2,6-P2 was examined in isolated rat hepatocytes. Glucagon (10-11 M), epinephrine (10-5 M), or calcium (2.4 mM) and A23187 (10-5 M) administration to hepatocytes produced simultaneous activation of fructose-2,6-Pase and inactivation of fructose-6-P,2-kinase within 2 minutes. The effect of epinephrine on these two enzymes was dependent on the presence of Ca++. These results suggest that the level of fructose-2,6-P2 is controlled by recriprocal changes in fructose-2,6-Pase and fructose-6-P,2-kinase activities.

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Reciprocal changes in fructose-6-phosphate,2-kinase and fructose-2,6-bisphosphatase activity in response to glucagon and epinephrine

Abstract

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