Reciprocal efficiency of RNQ1 and polyglutamine detoxification in the cytosol and nucleus

Peter M. Douglas, Daniel W. Summers, Hong Yu Ren, Douglas M. Cyr

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Onset of proteotoxicity is linked to change in the subcellular location of proteins that cause misfolding diseases. Yet, factors that drive changes in disease protein localization and the impact of residence in new surroundings on proteotoxicity are not entirely clear. To address these issues, we examined aspects of proteotoxicity caused by Rnq1-green fluorescent protein (GFP) and a huntingtin's protein exon-1 fragment with an expanded polyglutamine tract (Htt-103Q), which is dependent upon the intracellular presence of [RNQ +] prions. Increasing heat-shock protein 40 chaperone activity before Rnq1-GFP expression, shifted Rnq1-GFP aggregation from the cytosol to the nucleus. Assembly of Rnq1-GFP into benign amyloid-like aggregates was more efficient in the nucleus than cytosol and nuclear accumulation of Rnq1-GFP correlated with reduced toxicity. [RNQ+] prions were found to form stable complexes with Htt-103Q, and nuclear Rnq1-GFP aggregates were capable of sequestering Htt-103Q in the nucleus. On accumulation in the nucleus, conversion of Htt-103Q into SDS-resistant aggregates was dramatically reduced and Htt-103Q toxicity was exacerbated. Alterations in activity of molecular chaperones, the localization of intracellular interaction partners, or both can impact the cellular location of disease proteins. This, in turn, impacts proteotoxicity because the assembly of proteins to a benign state occurs with different efficiencies in the cytosol and nucleus.

Original languageEnglish (US)
Pages (from-to)4162-4173
Number of pages12
JournalMolecular Biology of the Cell
Volume20
Issue number19
DOIs
StatePublished - Oct 1 2009

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Green Fluorescent Proteins
Cytosol
Prions
HSP40 Heat-Shock Proteins
Proteostasis Deficiencies
Proteins
Molecular Chaperones
Amyloid
polyglutamine
Exons

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

Cite this

Reciprocal efficiency of RNQ1 and polyglutamine detoxification in the cytosol and nucleus. / Douglas, Peter M.; Summers, Daniel W.; Ren, Hong Yu; Cyr, Douglas M.

In: Molecular Biology of the Cell, Vol. 20, No. 19, 01.10.2009, p. 4162-4173.

Research output: Contribution to journalArticle

Douglas, Peter M. ; Summers, Daniel W. ; Ren, Hong Yu ; Cyr, Douglas M. / Reciprocal efficiency of RNQ1 and polyglutamine detoxification in the cytosol and nucleus. In: Molecular Biology of the Cell. 2009 ; Vol. 20, No. 19. pp. 4162-4173.
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