TY - JOUR
T1 - Recognition and processing of misfolded proteins by PA700, the 19S regulatory complex of the 26S proteasome.
AU - Liu, Chang Wei
AU - Strickland, Elizabeth
AU - Demartino, George N.
AU - Thomas, Philip J.
PY - 2005
Y1 - 2005
N2 - The 26S proteasome is composed of the core 20S proteasome in association with the 19S regulatory complex, or PA700. PA700 has multiple activities, including ATPase activity, polyubiquitin-chain binding activity, deubiquitination activity, chaperone-like activity, and substrate remodeling activity. The concerted action of these activities leads to efficient degradation of protein substrates by the 26S proteasome. In this chapter we describe protocols for purifying PA700 and the 20S complexes from bovine red cells and present methods to assay the chaperone-like activity and the substrate remodeling activity of PA700.
AB - The 26S proteasome is composed of the core 20S proteasome in association with the 19S regulatory complex, or PA700. PA700 has multiple activities, including ATPase activity, polyubiquitin-chain binding activity, deubiquitination activity, chaperone-like activity, and substrate remodeling activity. The concerted action of these activities leads to efficient degradation of protein substrates by the 26S proteasome. In this chapter we describe protocols for purifying PA700 and the 20S complexes from bovine red cells and present methods to assay the chaperone-like activity and the substrate remodeling activity of PA700.
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M3 - Article
C2 - 15917627
AN - SCOPUS:20344404245
SN - 1064-3745
VL - 301
SP - 71
EP - 81
JO - Methods in molecular biology (Clifton, N.J.)
JF - Methods in molecular biology (Clifton, N.J.)
ER -