Recognition of chromosomal DNA inside cells by locked nucleic acids

Randall Beane, Sylvie Gabillet, Christophe Montaillier, Khalil Arar, David R. Corey

Research output: Contribution to journalArticlepeer-review

25 Scopus citations

Abstract

Sequence-selective recognition of DNA inside cells by oligonucleotides would provide valuable insights into cellular processes and new leads for therapeutics. Recent work, however, has shown that noncoding RNA transcripts overlap chromosomal DNA. These RNAs provide alternate targets for oligonucleotides designed to bind promoter DNA, potentially overturning previous assumptions about mechanism. Here, we show that antigene locked nucleic acids (agLNAs) reduce RNA levels of targeted genes, block RNA polymerase and transcription factor association at gene promoters, and bind to chromosomal DNA. These data suggest that the mechanism of LNAs involves recognition of chromosomal DNA and that LNAs are bona fide antigene molecules.

Original languageEnglish (US)
Pages (from-to)13147-13149
Number of pages3
JournalBiochemistry
Volume47
Issue number50
DOIs
StatePublished - Dec 16 2008

ASJC Scopus subject areas

  • Biochemistry

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