Recognition of chromosomal DNA inside cells by locked nucleic acids

Randall Beane, Sylvie Gabillet, Christophe Montaillier, Khalil Arar, David R. Corey

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Sequence-selective recognition of DNA inside cells by oligonucleotides would provide valuable insights into cellular processes and new leads for therapeutics. Recent work, however, has shown that noncoding RNA transcripts overlap chromosomal DNA. These RNAs provide alternate targets for oligonucleotides designed to bind promoter DNA, potentially overturning previous assumptions about mechanism. Here, we show that antigene locked nucleic acids (agLNAs) reduce RNA levels of targeted genes, block RNA polymerase and transcription factor association at gene promoters, and bind to chromosomal DNA. These data suggest that the mechanism of LNAs involves recognition of chromosomal DNA and that LNAs are bona fide antigene molecules.

Original languageEnglish (US)
Pages (from-to)13147-13149
Number of pages3
JournalBiochemistry
Volume47
Issue number50
DOIs
StatePublished - Dec 16 2008

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DNA
Oligonucleotides
Genes
RNA
Untranslated RNA
DNA-Directed RNA Polymerases
Transcription Factors
locked nucleic acid
Molecules
Therapeutics

ASJC Scopus subject areas

  • Biochemistry

Cite this

Recognition of chromosomal DNA inside cells by locked nucleic acids. / Beane, Randall; Gabillet, Sylvie; Montaillier, Christophe; Arar, Khalil; Corey, David R.

In: Biochemistry, Vol. 47, No. 50, 16.12.2008, p. 13147-13149.

Research output: Contribution to journalArticle

Beane, R, Gabillet, S, Montaillier, C, Arar, K & Corey, DR 2008, 'Recognition of chromosomal DNA inside cells by locked nucleic acids', Biochemistry, vol. 47, no. 50, pp. 13147-13149. https://doi.org/10.1021/bi801930p
Beane, Randall ; Gabillet, Sylvie ; Montaillier, Christophe ; Arar, Khalil ; Corey, David R. / Recognition of chromosomal DNA inside cells by locked nucleic acids. In: Biochemistry. 2008 ; Vol. 47, No. 50. pp. 13147-13149.
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