Recombinant antibody-metallothionein

Design and evaluation for radioimmunoimaging

C. Das, P. V. Kulkarni, A. Constantinescu, P. Antich, F. R. Blattner, P. W. Tucker

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

We have produced a chimeric antibody (Ab) in which metallothionein, a well-characterized biological chelator of metals, was genetically fused to the F(ab′) domain of the S107 Ab heavy chain. Coexpression with the Ab light chain that conveys specificity for the synthetic antigen phosphocholine was achieved in plasmacytoma cells. Metal- and antigen-binding domains of the Ab-metallothionein hybrid function with normal avidity and specificity. Ab-metallothionein can be efficiently loaded with 99mTc and used to specifically bind phosphocholine-haptenated cells in vitro or to localize plasma-cell ascites tumors in mice. The approach offers potential advantages for producing radiolabeled Ab for targeted radiotherapy and diagnostic imaging.

Original languageEnglish (US)
Pages (from-to)9749-9753
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume89
Issue number20
StatePublished - Oct 15 1992

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Radioimmunodetection
Metallothionein
Antibodies
Plasmacytoma
Phosphorylcholine
Metals
Synthetic Vaccines
Diagnostic Imaging
Chelating Agents
Ascites
Radiotherapy
Light
Antigens

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

Recombinant antibody-metallothionein : Design and evaluation for radioimmunoimaging. / Das, C.; Kulkarni, P. V.; Constantinescu, A.; Antich, P.; Blattner, F. R.; Tucker, P. W.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 89, No. 20, 15.10.1992, p. 9749-9753.

Research output: Contribution to journalArticle

Das, C. ; Kulkarni, P. V. ; Constantinescu, A. ; Antich, P. ; Blattner, F. R. ; Tucker, P. W. / Recombinant antibody-metallothionein : Design and evaluation for radioimmunoimaging. In: Proceedings of the National Academy of Sciences of the United States of America. 1992 ; Vol. 89, No. 20. pp. 9749-9753.
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