Recombinant interleukin 1 suppresses lipoprotein lipase activity in 3T3-L1 cells

B. A. Beutler, A. Cerami

Research output: Contribution to journalArticle

172 Citations (Scopus)

Abstract

Recombinant murine interleukin 1 (rIL 1) inhibits 3T3-L1 cell expression of lipoprotein lipase (LPL) activity when present in exceedingly dilute concentration (<10-15 M). The extreme sensitivity of the adipocyte system to rIL 1 far exceeds that of the standard lymphocyte-activating factor assay. However, enzyme suppression is incomplete; even at micromolar concentrations, rIL 1 causes only about a 50% reduction in LPL activity. By contrast, cachectin (tumour necrosis factor) achieves nearly complete LPL suppression at subnanomolar concentrations. Concentrated solutions of rIL 1 are incapable of competing with radiolabeled cachectin for binding sites on 3T3-L1 cells. rIL 1-induced LPL suppression is abolished by the addition of a specific IL 1 neutralizing antiserum to the assay system. rIL 1 appears capable of influencing adipocyte expression of LPL, but apparently acts through a different mechanism than cachectin/TNF.

Original languageEnglish (US)
Pages (from-to)3969-3971
Number of pages3
JournalJournal of Immunology
Volume135
Issue number6
StatePublished - 1985

Fingerprint

3T3-L1 Cells
Lipoprotein Lipase
Interleukin-1
Tumor Necrosis Factor-alpha
Adipocytes
Immune Sera
Binding Sites

ASJC Scopus subject areas

  • Immunology

Cite this

Recombinant interleukin 1 suppresses lipoprotein lipase activity in 3T3-L1 cells. / Beutler, B. A.; Cerami, A.

In: Journal of Immunology, Vol. 135, No. 6, 1985, p. 3969-3971.

Research output: Contribution to journalArticle

@article{40923bedfbd24694a3a8636ed5b6efdb,
title = "Recombinant interleukin 1 suppresses lipoprotein lipase activity in 3T3-L1 cells",
abstract = "Recombinant murine interleukin 1 (rIL 1) inhibits 3T3-L1 cell expression of lipoprotein lipase (LPL) activity when present in exceedingly dilute concentration (<10-15 M). The extreme sensitivity of the adipocyte system to rIL 1 far exceeds that of the standard lymphocyte-activating factor assay. However, enzyme suppression is incomplete; even at micromolar concentrations, rIL 1 causes only about a 50{\%} reduction in LPL activity. By contrast, cachectin (tumour necrosis factor) achieves nearly complete LPL suppression at subnanomolar concentrations. Concentrated solutions of rIL 1 are incapable of competing with radiolabeled cachectin for binding sites on 3T3-L1 cells. rIL 1-induced LPL suppression is abolished by the addition of a specific IL 1 neutralizing antiserum to the assay system. rIL 1 appears capable of influencing adipocyte expression of LPL, but apparently acts through a different mechanism than cachectin/TNF.",
author = "Beutler, {B. A.} and A. Cerami",
year = "1985",
language = "English (US)",
volume = "135",
pages = "3969--3971",
journal = "Journal of Immunology",
issn = "0022-1767",
publisher = "American Association of Immunologists",
number = "6",

}

TY - JOUR

T1 - Recombinant interleukin 1 suppresses lipoprotein lipase activity in 3T3-L1 cells

AU - Beutler, B. A.

AU - Cerami, A.

PY - 1985

Y1 - 1985

N2 - Recombinant murine interleukin 1 (rIL 1) inhibits 3T3-L1 cell expression of lipoprotein lipase (LPL) activity when present in exceedingly dilute concentration (<10-15 M). The extreme sensitivity of the adipocyte system to rIL 1 far exceeds that of the standard lymphocyte-activating factor assay. However, enzyme suppression is incomplete; even at micromolar concentrations, rIL 1 causes only about a 50% reduction in LPL activity. By contrast, cachectin (tumour necrosis factor) achieves nearly complete LPL suppression at subnanomolar concentrations. Concentrated solutions of rIL 1 are incapable of competing with radiolabeled cachectin for binding sites on 3T3-L1 cells. rIL 1-induced LPL suppression is abolished by the addition of a specific IL 1 neutralizing antiserum to the assay system. rIL 1 appears capable of influencing adipocyte expression of LPL, but apparently acts through a different mechanism than cachectin/TNF.

AB - Recombinant murine interleukin 1 (rIL 1) inhibits 3T3-L1 cell expression of lipoprotein lipase (LPL) activity when present in exceedingly dilute concentration (<10-15 M). The extreme sensitivity of the adipocyte system to rIL 1 far exceeds that of the standard lymphocyte-activating factor assay. However, enzyme suppression is incomplete; even at micromolar concentrations, rIL 1 causes only about a 50% reduction in LPL activity. By contrast, cachectin (tumour necrosis factor) achieves nearly complete LPL suppression at subnanomolar concentrations. Concentrated solutions of rIL 1 are incapable of competing with radiolabeled cachectin for binding sites on 3T3-L1 cells. rIL 1-induced LPL suppression is abolished by the addition of a specific IL 1 neutralizing antiserum to the assay system. rIL 1 appears capable of influencing adipocyte expression of LPL, but apparently acts through a different mechanism than cachectin/TNF.

UR - http://www.scopus.com/inward/record.url?scp=0022202322&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0022202322&partnerID=8YFLogxK

M3 - Article

VL - 135

SP - 3969

EP - 3971

JO - Journal of Immunology

JF - Journal of Immunology

SN - 0022-1767

IS - 6

ER -