Reconstitution of catecholamine-sensitive adenylate cyclase activity: interaction of solubilized components with receptor-replete membranes

E. M. Ross, A. G. Gilman

Research output: Contribution to journalArticle

101 Scopus citations

Abstract

Membranes of mouse L cells which contain adenylate cyclase [ATP pyrophosphate-lyase (cyclizing)] but lack β adrenergic receptors were solubilized with Lubrol 12A9. Addition of such adenylate cyclase containing extracts to β adrenergic receptor replete membranes from adenylate cyclase deficient S49 lymphoma cells results in the production of a catecholamine sensitive adenylate cyclase system. The effects of β adrenergic agonists and antagonists on the reconstituted system reproduce those that are characteristic of the wild type S49 lymphoma cell. The uncoupled variant of the S49 lymphoma contains adenylate cyclase, but donor extracts from this clone fail to reconstitute the hormone sensitive enzyme activity when added to adenylate cyclase deficient membranes. Thus, the uncoupled and adenylate cyclase deficient variants of the S49 cell are not complementary.

Original languageEnglish (US)
Pages (from-to)3715-3719
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume74
Issue number9
DOIs
StatePublished - Jan 1 1977

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