TY - JOUR
T1 - Reconstitution of endoplasmic reticulum InsP3 receptors into black lipid membranes
AU - Bezprozvanny, Ilya
PY - 2013/11
Y1 - 2013/11
N2 - Intracellular ion channels, including endoplasmic reticulum (ER) calcium (Ca2+) channels, are most often studied through their reconstitution into planar lipid bilayers (also called black lipid membranes, or BLMs). General methods for making bilayers and for ion channel reconstitution into BLMs have been extensively detailed elsewhere; thus, here the focus is on specific details relevant for inositol (1,4,5)-trisphosphate receptor (InsP3) recordings. These procedures describe how to perform single-channel recordings of native or recombinant InsP3s in BLMs. Similar procedures are used to study native or recombinant ryanodine receptors (RyanRs) in BLMs.
AB - Intracellular ion channels, including endoplasmic reticulum (ER) calcium (Ca2+) channels, are most often studied through their reconstitution into planar lipid bilayers (also called black lipid membranes, or BLMs). General methods for making bilayers and for ion channel reconstitution into BLMs have been extensively detailed elsewhere; thus, here the focus is on specific details relevant for inositol (1,4,5)-trisphosphate receptor (InsP3) recordings. These procedures describe how to perform single-channel recordings of native or recombinant InsP3s in BLMs. Similar procedures are used to study native or recombinant ryanodine receptors (RyanRs) in BLMs.
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U2 - 10.1101/pdb.prot073106
DO - 10.1101/pdb.prot073106
M3 - Article
C2 - 24184757
AN - SCOPUS:84887106833
SN - 1940-3402
VL - 2013
SP - 1041
EP - 1047
JO - Cold Spring Harbor Protocols
JF - Cold Spring Harbor Protocols
IS - 11
ER -