Reconstitution of endoplasmic reticulum InsP3 receptors into black lipid membranes

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Abstract

Intracellular ion channels, including endoplasmic reticulum (ER) calcium (Ca2+) channels, are most often studied through their reconstitution into planar lipid bilayers (also called black lipid membranes, or BLMs). General methods for making bilayers and for ion channel reconstitution into BLMs have been extensively detailed elsewhere; thus, here the focus is on specific details relevant for inositol (1,4,5)-trisphosphate receptor (InsP3) recordings. These procedures describe how to perform single-channel recordings of native or recombinant InsP3s in BLMs. Similar procedures are used to study native or recombinant ryanodine receptors (RyanRs) in BLMs.

Original languageEnglish (US)
Pages (from-to)1041-1047
Number of pages7
JournalCold Spring Harbor Protocols
Volume2013
Issue number11
DOIs
StatePublished - Nov 2013

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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