Reconstruction of catecholamine-sensitive adenylate cyclase activity: interaction of components following cell-cell and membrane-cell fusion

J. D. Schwartzmeier, A. G. Gilman

Research output: Contribution to journalArticle

13 Scopus citations

Abstract

Heterokaryons formed by the fusion of adenylate cyclase-deficient S49 cells and β-adrenergic receptor-deficient B82 cells in the presence of cycloheximide display a catecholamine-sensitive adenylate cyclase activity. Similar complementation can be observed when receptor-replete membranes from the cyclase-deficient cell are fused with intact B82 cells. Using the cell fusion technique it can be demonstrated that the uncoupled S49 cell variant has a functional β-adrenergic receptor but that this variant and the adenylate cyclase-deficient variant are not complementary. Hypothetically, both clones may share a common defect in regulatory components of adenylate cyclase or both may lack a specific coupling factor.

Original languageEnglish (US)
Pages (from-to)227-238
Number of pages12
JournalJournal of Cyclic Nucleotide Research
Volume3
Issue number4
StatePublished - Jan 1 1977

ASJC Scopus subject areas

  • Medicine(all)

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