Recruitment of Atg9 to the preautophagosomal structure by Atg11 is essential for selective autophagy in budding yeast

Congcong He, Hui Song, Tomohiro Yorimitsu, Iryna Monastyrska, Wei Lien Yen, Julie E. Legakis, Daniel J. Klionsky

Research output: Contribution to journalArticle

120 Citations (Scopus)

Abstract

Autophagy is a conserved degradative pathway that is induced in response to various stress and develop mental conditions in eukaryotic cells. It allows the elimination of cytosolic proteins and organelles in the lysosome/vacuole. In the yeast Saccharomyces cerevisiae, the integral membrane protein Atg9 (autophagy-related protein 9) cycles between mitochondria and the preautophagosomal structure (PAS), the nucleating site for formation of the sequestering vesicle, suggesting a role in supplying membrane for vesicle formation and/or expansion during autophagy. To better understand the mechanisms involved in Atg9 cycling, we performed a yeast two-hybrid-based screen and identified a peripheral membrane protein, Atg11, that interacts with Atg9. We show that Atg11 governs Atg9 cycling through the PAS during specific autophagy. We also demonstrate that the integrity of the actin cytoskeleton is essential for correct targeting of Atg11 to the PAS. We propose that a pool of Atg11 mediates the anterograde transport of Atg9 to the PAS that is dependent on the actin cytoskeleton during yeast vegetative growth.

Original languageEnglish (US)
Pages (from-to)925-935
Number of pages11
JournalJournal of Cell Biology
Volume175
Issue number6
DOIs
StatePublished - Dec 18 2006

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Saccharomycetales
Autophagy
Yeasts
Actin Cytoskeleton
Membrane Proteins
Eukaryotic Cells
Vacuoles
Lysosomes
Organelles
Saccharomyces cerevisiae
Mitochondria
Autophagy-Related Proteins
Membranes
Growth
Proteins

ASJC Scopus subject areas

  • Cell Biology

Cite this

He, C., Song, H., Yorimitsu, T., Monastyrska, I., Yen, W. L., Legakis, J. E., & Klionsky, D. J. (2006). Recruitment of Atg9 to the preautophagosomal structure by Atg11 is essential for selective autophagy in budding yeast. Journal of Cell Biology, 175(6), 925-935. https://doi.org/10.1083/jcb.200606084

Recruitment of Atg9 to the preautophagosomal structure by Atg11 is essential for selective autophagy in budding yeast. / He, Congcong; Song, Hui; Yorimitsu, Tomohiro; Monastyrska, Iryna; Yen, Wei Lien; Legakis, Julie E.; Klionsky, Daniel J.

In: Journal of Cell Biology, Vol. 175, No. 6, 18.12.2006, p. 925-935.

Research output: Contribution to journalArticle

He, C, Song, H, Yorimitsu, T, Monastyrska, I, Yen, WL, Legakis, JE & Klionsky, DJ 2006, 'Recruitment of Atg9 to the preautophagosomal structure by Atg11 is essential for selective autophagy in budding yeast', Journal of Cell Biology, vol. 175, no. 6, pp. 925-935. https://doi.org/10.1083/jcb.200606084
He, Congcong ; Song, Hui ; Yorimitsu, Tomohiro ; Monastyrska, Iryna ; Yen, Wei Lien ; Legakis, Julie E. ; Klionsky, Daniel J. / Recruitment of Atg9 to the preautophagosomal structure by Atg11 is essential for selective autophagy in budding yeast. In: Journal of Cell Biology. 2006 ; Vol. 175, No. 6. pp. 925-935.
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