Red blood cells

Reservoirs of cis- and trans-epoxyeicosatrienoic acids

Houli Jiang, John Quilley, L. Manmohan Reddy, John R. Falck, Patrick Y K Wong, John C. McGiff

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Epoxyeicosatrienoic acids (EETs) are candidate endothelium-derived hyperpolarizing factors that demonstrate a wide range of biological effects. The presence of both cis- and trans-EETs in rat plasma was identified with HPLC-electrospray ionization tandem mass spectrometry in this study. The total EETs in plasma are 38.2 ng/ml with cis-EETs representing 21.4 ± 0.4 ng/ml and trans-EETs 16.8 ± 0.4 ng/ml. EETs in RBCs were estimated to be 20.2 ng/109 RBCs, which corresponds to 200 ng in RBCs contained in 1 ml blood. RBC incubation with 10 mM tert-butyl hydroperoxide resulted in 4.4-fold increase of total cis-EETs (from 9.2 to 40.2 ng/109 RBCs) and 5.5-fold increase of total trans-EETs (from 11.0 to 60.8 ng/109 RBCs). EETs were released (2 ng/ml) from RBCs after incubation at 37°C for 10 min even after being washed 3 times, indicating that RBCs are reservoirs of plasma EETs. The identification of cis- and trans-EETs in RBCs and in plasma as well as their release from RBCs suggest a vasoregulatory role of RBCs in view of their potent vasoactivity.

Original languageEnglish (US)
Pages (from-to)65-78
Number of pages14
JournalProstaglandins and Other Lipid Mediators
Volume75
Issue number1-4
DOIs
StatePublished - Jan 2005

Fingerprint

Blood
Erythrocytes
Cells
Plasmas
Acids
tert-Butylhydroperoxide
Electrospray ionization
Electrospray Ionization Mass Spectrometry
Tandem Mass Spectrometry
Endothelium
Mass spectrometry
Rats
High Pressure Liquid Chromatography

Keywords

  • Epoxyeicosatrienoic acid
  • Peroxidation
  • Plasma
  • Red blood cells

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology

Cite this

Red blood cells : Reservoirs of cis- and trans-epoxyeicosatrienoic acids. / Jiang, Houli; Quilley, John; Reddy, L. Manmohan; Falck, John R.; Wong, Patrick Y K; McGiff, John C.

In: Prostaglandins and Other Lipid Mediators, Vol. 75, No. 1-4, 01.2005, p. 65-78.

Research output: Contribution to journalArticle

Jiang, Houli ; Quilley, John ; Reddy, L. Manmohan ; Falck, John R. ; Wong, Patrick Y K ; McGiff, John C. / Red blood cells : Reservoirs of cis- and trans-epoxyeicosatrienoic acids. In: Prostaglandins and Other Lipid Mediators. 2005 ; Vol. 75, No. 1-4. pp. 65-78.
@article{7ccc25a7e4d74570ab873a59a0d2a664,
title = "Red blood cells: Reservoirs of cis- and trans-epoxyeicosatrienoic acids",
abstract = "Epoxyeicosatrienoic acids (EETs) are candidate endothelium-derived hyperpolarizing factors that demonstrate a wide range of biological effects. The presence of both cis- and trans-EETs in rat plasma was identified with HPLC-electrospray ionization tandem mass spectrometry in this study. The total EETs in plasma are 38.2 ng/ml with cis-EETs representing 21.4 ± 0.4 ng/ml and trans-EETs 16.8 ± 0.4 ng/ml. EETs in RBCs were estimated to be 20.2 ng/109 RBCs, which corresponds to 200 ng in RBCs contained in 1 ml blood. RBC incubation with 10 mM tert-butyl hydroperoxide resulted in 4.4-fold increase of total cis-EETs (from 9.2 to 40.2 ng/109 RBCs) and 5.5-fold increase of total trans-EETs (from 11.0 to 60.8 ng/109 RBCs). EETs were released (2 ng/ml) from RBCs after incubation at 37°C for 10 min even after being washed 3 times, indicating that RBCs are reservoirs of plasma EETs. The identification of cis- and trans-EETs in RBCs and in plasma as well as their release from RBCs suggest a vasoregulatory role of RBCs in view of their potent vasoactivity.",
keywords = "Epoxyeicosatrienoic acid, Peroxidation, Plasma, Red blood cells",
author = "Houli Jiang and John Quilley and Reddy, {L. Manmohan} and Falck, {John R.} and Wong, {Patrick Y K} and McGiff, {John C.}",
year = "2005",
month = "1",
doi = "10.1016/j.prostaglandins.2004.10.003",
language = "English (US)",
volume = "75",
pages = "65--78",
journal = "Prostaglandins and Other Lipid Mediators",
issn = "1098-8823",
publisher = "Elsevier BV",
number = "1-4",

}

TY - JOUR

T1 - Red blood cells

T2 - Reservoirs of cis- and trans-epoxyeicosatrienoic acids

AU - Jiang, Houli

AU - Quilley, John

AU - Reddy, L. Manmohan

AU - Falck, John R.

AU - Wong, Patrick Y K

AU - McGiff, John C.

PY - 2005/1

Y1 - 2005/1

N2 - Epoxyeicosatrienoic acids (EETs) are candidate endothelium-derived hyperpolarizing factors that demonstrate a wide range of biological effects. The presence of both cis- and trans-EETs in rat plasma was identified with HPLC-electrospray ionization tandem mass spectrometry in this study. The total EETs in plasma are 38.2 ng/ml with cis-EETs representing 21.4 ± 0.4 ng/ml and trans-EETs 16.8 ± 0.4 ng/ml. EETs in RBCs were estimated to be 20.2 ng/109 RBCs, which corresponds to 200 ng in RBCs contained in 1 ml blood. RBC incubation with 10 mM tert-butyl hydroperoxide resulted in 4.4-fold increase of total cis-EETs (from 9.2 to 40.2 ng/109 RBCs) and 5.5-fold increase of total trans-EETs (from 11.0 to 60.8 ng/109 RBCs). EETs were released (2 ng/ml) from RBCs after incubation at 37°C for 10 min even after being washed 3 times, indicating that RBCs are reservoirs of plasma EETs. The identification of cis- and trans-EETs in RBCs and in plasma as well as their release from RBCs suggest a vasoregulatory role of RBCs in view of their potent vasoactivity.

AB - Epoxyeicosatrienoic acids (EETs) are candidate endothelium-derived hyperpolarizing factors that demonstrate a wide range of biological effects. The presence of both cis- and trans-EETs in rat plasma was identified with HPLC-electrospray ionization tandem mass spectrometry in this study. The total EETs in plasma are 38.2 ng/ml with cis-EETs representing 21.4 ± 0.4 ng/ml and trans-EETs 16.8 ± 0.4 ng/ml. EETs in RBCs were estimated to be 20.2 ng/109 RBCs, which corresponds to 200 ng in RBCs contained in 1 ml blood. RBC incubation with 10 mM tert-butyl hydroperoxide resulted in 4.4-fold increase of total cis-EETs (from 9.2 to 40.2 ng/109 RBCs) and 5.5-fold increase of total trans-EETs (from 11.0 to 60.8 ng/109 RBCs). EETs were released (2 ng/ml) from RBCs after incubation at 37°C for 10 min even after being washed 3 times, indicating that RBCs are reservoirs of plasma EETs. The identification of cis- and trans-EETs in RBCs and in plasma as well as their release from RBCs suggest a vasoregulatory role of RBCs in view of their potent vasoactivity.

KW - Epoxyeicosatrienoic acid

KW - Peroxidation

KW - Plasma

KW - Red blood cells

UR - http://www.scopus.com/inward/record.url?scp=13844250387&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=13844250387&partnerID=8YFLogxK

U2 - 10.1016/j.prostaglandins.2004.10.003

DO - 10.1016/j.prostaglandins.2004.10.003

M3 - Article

VL - 75

SP - 65

EP - 78

JO - Prostaglandins and Other Lipid Mediators

JF - Prostaglandins and Other Lipid Mediators

SN - 1098-8823

IS - 1-4

ER -