Red blood cells: Reservoirs of cis- and trans-epoxyeicosatrienoic acids

Houli Jiang; John Quilley; L. Manmohan Reddy; John R. Falck; Patrick Y K Wong; John C. McGiff

doi:10.1016/j.prostaglandins.2004.10.003

Red blood cells: Reservoirs of cis- and trans-epoxyeicosatrienoic acids

Houli Jiang, John Quilley, L. Manmohan Reddy, John R. Falck, Patrick Y K Wong, John C. McGiff

Biochemistry

Research output: Contribution to journal › Article › peer-review

29 Scopus citations

Abstract

Epoxyeicosatrienoic acids (EETs) are candidate endothelium-derived hyperpolarizing factors that demonstrate a wide range of biological effects. The presence of both cis- and trans-EETs in rat plasma was identified with HPLC-electrospray ionization tandem mass spectrometry in this study. The total EETs in plasma are 38.2 ng/ml with cis-EETs representing 21.4 ± 0.4 ng/ml and trans-EETs 16.8 ± 0.4 ng/ml. EETs in RBCs were estimated to be 20.2 ng/10⁹ RBCs, which corresponds to 200 ng in RBCs contained in 1 ml blood. RBC incubation with 10 mM tert-butyl hydroperoxide resulted in 4.4-fold increase of total cis-EETs (from 9.2 to 40.2 ng/10⁹ RBCs) and 5.5-fold increase of total trans-EETs (from 11.0 to 60.8 ng/10⁹ RBCs). EETs were released (2 ng/ml) from RBCs after incubation at 37°C for 10 min even after being washed 3 times, indicating that RBCs are reservoirs of plasma EETs. The identification of cis- and trans-EETs in RBCs and in plasma as well as their release from RBCs suggest a vasoregulatory role of RBCs in view of their potent vasoactivity.

Original language	English (US)
Pages (from-to)	65-78
Number of pages	14
Journal	Prostaglandins and Other Lipid Mediators
Volume	75
Issue number	1-4
DOIs	https://doi.org/10.1016/j.prostaglandins.2004.10.003
State	Published - Jan 2005

Keywords

Epoxyeicosatrienoic acid
Peroxidation
Plasma
Red blood cells

ASJC Scopus subject areas

Biochemistry
Physiology
Pharmacology
Cell Biology

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10.1016/j.prostaglandins.2004.10.003

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title = "Red blood cells: Reservoirs of cis- and trans-epoxyeicosatrienoic acids",

abstract = "Epoxyeicosatrienoic acids (EETs) are candidate endothelium-derived hyperpolarizing factors that demonstrate a wide range of biological effects. The presence of both cis- and trans-EETs in rat plasma was identified with HPLC-electrospray ionization tandem mass spectrometry in this study. The total EETs in plasma are 38.2 ng/ml with cis-EETs representing 21.4 ± 0.4 ng/ml and trans-EETs 16.8 ± 0.4 ng/ml. EETs in RBCs were estimated to be 20.2 ng/109 RBCs, which corresponds to 200 ng in RBCs contained in 1 ml blood. RBC incubation with 10 mM tert-butyl hydroperoxide resulted in 4.4-fold increase of total cis-EETs (from 9.2 to 40.2 ng/109 RBCs) and 5.5-fold increase of total trans-EETs (from 11.0 to 60.8 ng/109 RBCs). EETs were released (2 ng/ml) from RBCs after incubation at 37°C for 10 min even after being washed 3 times, indicating that RBCs are reservoirs of plasma EETs. The identification of cis- and trans-EETs in RBCs and in plasma as well as their release from RBCs suggest a vasoregulatory role of RBCs in view of their potent vasoactivity.",

keywords = "Epoxyeicosatrienoic acid, Peroxidation, Plasma, Red blood cells",

author = "Houli Jiang and John Quilley and Reddy, {L. Manmohan} and Falck, {John R.} and Wong, {Patrick Y K} and McGiff, {John C.}",

note = "Funding Information: This investigation was supported by National Institutes of Health Grants HL-25394 (JCM), HL-34300 (JCM), GM31278 (JRF), the Robert A. Welch Foundation (JRF) and an American Diabetes Association Grant (JQ).",

year = "2005",

month = jan,

doi = "10.1016/j.prostaglandins.2004.10.003",

language = "English (US)",

volume = "75",

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publisher = "Elsevier BV",

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T2 - Reservoirs of cis- and trans-epoxyeicosatrienoic acids

AU - Jiang, Houli

AU - Quilley, John

AU - Reddy, L. Manmohan

AU - Falck, John R.

AU - Wong, Patrick Y K

AU - McGiff, John C.

N1 - Funding Information: This investigation was supported by National Institutes of Health Grants HL-25394 (JCM), HL-34300 (JCM), GM31278 (JRF), the Robert A. Welch Foundation (JRF) and an American Diabetes Association Grant (JQ).

PY - 2005/1

Y1 - 2005/1

N2 - Epoxyeicosatrienoic acids (EETs) are candidate endothelium-derived hyperpolarizing factors that demonstrate a wide range of biological effects. The presence of both cis- and trans-EETs in rat plasma was identified with HPLC-electrospray ionization tandem mass spectrometry in this study. The total EETs in plasma are 38.2 ng/ml with cis-EETs representing 21.4 ± 0.4 ng/ml and trans-EETs 16.8 ± 0.4 ng/ml. EETs in RBCs were estimated to be 20.2 ng/109 RBCs, which corresponds to 200 ng in RBCs contained in 1 ml blood. RBC incubation with 10 mM tert-butyl hydroperoxide resulted in 4.4-fold increase of total cis-EETs (from 9.2 to 40.2 ng/109 RBCs) and 5.5-fold increase of total trans-EETs (from 11.0 to 60.8 ng/109 RBCs). EETs were released (2 ng/ml) from RBCs after incubation at 37°C for 10 min even after being washed 3 times, indicating that RBCs are reservoirs of plasma EETs. The identification of cis- and trans-EETs in RBCs and in plasma as well as their release from RBCs suggest a vasoregulatory role of RBCs in view of their potent vasoactivity.

AB - Epoxyeicosatrienoic acids (EETs) are candidate endothelium-derived hyperpolarizing factors that demonstrate a wide range of biological effects. The presence of both cis- and trans-EETs in rat plasma was identified with HPLC-electrospray ionization tandem mass spectrometry in this study. The total EETs in plasma are 38.2 ng/ml with cis-EETs representing 21.4 ± 0.4 ng/ml and trans-EETs 16.8 ± 0.4 ng/ml. EETs in RBCs were estimated to be 20.2 ng/109 RBCs, which corresponds to 200 ng in RBCs contained in 1 ml blood. RBC incubation with 10 mM tert-butyl hydroperoxide resulted in 4.4-fold increase of total cis-EETs (from 9.2 to 40.2 ng/109 RBCs) and 5.5-fold increase of total trans-EETs (from 11.0 to 60.8 ng/109 RBCs). EETs were released (2 ng/ml) from RBCs after incubation at 37°C for 10 min even after being washed 3 times, indicating that RBCs are reservoirs of plasma EETs. The identification of cis- and trans-EETs in RBCs and in plasma as well as their release from RBCs suggest a vasoregulatory role of RBCs in view of their potent vasoactivity.

KW - Epoxyeicosatrienoic acid

KW - Peroxidation

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KW - Red blood cells

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JO - Prostaglandins and Other Lipid Mediators

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IS - 1-4

ER -

Red blood cells: Reservoirs of cis- and trans-epoxyeicosatrienoic acids

Abstract

Keywords

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