Reduced β-cell glucose transporter in new onset diabetic BB rats

L. Orci, Roger H Unger, M. Ravazzola, A. Ogawa, I. Komiya, Dany Baetens, H. F. Lodish, B. Thorens

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Abstract

Previous studies from our laboratories have suggested a defect in glucose transport in islets isolated from BB rats on the first day of overt diabetes. To quantitate by immunostaining the glucose transporter of β-cells (GLUT-2) before and at the onset of autoimmune diabetes we employed an antibody to its COOH-terminal octapeptide. On the first day of over diabetes, defined as the day the daily blood glucose first reached 200 mg/dl, the volume density ratio of GLUT-2-positive to insulin-positive β-cells was only 0.48 ± 0.06, compared to 0.91 ± 0.02 in age-matched nondiabetic diabetes-resistant controls (P < 0.001). In age-matched nondiabetic diabetes-prone rats, most of which would have become diabetic, the ratio was 0.85 ± 0.02, also less than the controls (P < 0.05). Protein A-gold labelling of GLUT-2 in β-cells of day 1 diabetic rats revealed 2.17 ± 0.16 gold particles per micrometer length of microvillar plasma membranes compared to 3.91 ± 0.14 in controls (P < 0.001) and 2.87 ± 0.24 in the nondiabetic diabetes-prone rats (P < 0.02). Reduction in GLUT-2 correlates temporally with and may contribute to the loss of glucose-stimulated insulin secretion that precedes profound β-cell depletion of autoimmune diabetes.

Original languageEnglish (US)
Pages (from-to)1615-1622
Number of pages8
JournalJournal of Clinical Investigation
Volume86
Issue number5
DOIs
StatePublished - 1990

Keywords

  • glucose transporter
  • glucose-stimulated insulin secretion
  • homeostasis

ASJC Scopus subject areas

  • Medicine(all)

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