Reduced β-cell glucose transporter in new onset diabetic BB rats

L. Orci, Roger H Unger, M. Ravazzola, A. Ogawa, I. Komiya, Dany Baetens, H. F. Lodish, B. Thorens

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Abstract

Previous studies from our laboratories have suggested a defect in glucose transport in islets isolated from BB rats on the first day of overt diabetes. To quantitate by immunostaining the glucose transporter of β-cells (GLUT-2) before and at the onset of autoimmune diabetes we employed an antibody to its COOH-terminal octapeptide. On the first day of overt diabetes, defined as the day the daily blood glucose first reached 200 mg/dl, the volume density ratio of GLUT-2-positive to insulin-positive β-cells was only 0.48±0.06, compared to 0.91±0.02 in age-matched nondiabetic diabetes-resistant controls (P<0.001). In age-matched nondiabetic diabetes-prone rats, most of which would have become diabetic, the ratio was 0.85±0.02, also less than the controls (P<0.05). Protein A-gold labeling of GLUT-2 in β-cells of day 1 diabetic rats revealed 2.17±0.16 gold particles per micrometer length of microvillar plasma membranes compared to 3.91±0.14 in controls (P<0.001) and 2.87±0.24 in the nondiabetic diabetes-prone rats (P<0.02). Reduction in GLUT-2 correlates temporally with and may contribute to the loss of glucose-stimulated insulin secretion that precedes profound β-cell depletion of autoimmune diabetes.

Original languageEnglish (US)
Pages (from-to)1615-1622
Number of pages8
JournalJournal of Clinical Investigation
Volume86
Issue number5
StatePublished - Nov 1990

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Inbred BB Rats
Facilitative Glucose Transport Proteins
Type 1 Diabetes Mellitus
Gold
Insulin
Glucose
Staphylococcal Protein A
Blood Glucose
Cell Membrane
Antibodies

Keywords

  • Glucose transporter
  • Glucose-stimulated insulin secretion
  • Homeostasis

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Orci, L., Unger, R. H., Ravazzola, M., Ogawa, A., Komiya, I., Baetens, D., ... Thorens, B. (1990). Reduced β-cell glucose transporter in new onset diabetic BB rats. Journal of Clinical Investigation, 86(5), 1615-1622.

Reduced β-cell glucose transporter in new onset diabetic BB rats. / Orci, L.; Unger, Roger H; Ravazzola, M.; Ogawa, A.; Komiya, I.; Baetens, Dany; Lodish, H. F.; Thorens, B.

In: Journal of Clinical Investigation, Vol. 86, No. 5, 11.1990, p. 1615-1622.

Research output: Contribution to journalArticle

Orci, L, Unger, RH, Ravazzola, M, Ogawa, A, Komiya, I, Baetens, D, Lodish, HF & Thorens, B 1990, 'Reduced β-cell glucose transporter in new onset diabetic BB rats', Journal of Clinical Investigation, vol. 86, no. 5, pp. 1615-1622.
Orci L, Unger RH, Ravazzola M, Ogawa A, Komiya I, Baetens D et al. Reduced β-cell glucose transporter in new onset diabetic BB rats. Journal of Clinical Investigation. 1990 Nov;86(5):1615-1622.
Orci, L. ; Unger, Roger H ; Ravazzola, M. ; Ogawa, A. ; Komiya, I. ; Baetens, Dany ; Lodish, H. F. ; Thorens, B. / Reduced β-cell glucose transporter in new onset diabetic BB rats. In: Journal of Clinical Investigation. 1990 ; Vol. 86, No. 5. pp. 1615-1622.
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