TY - JOUR
T1 - Reduced binding of protein phosphatase 2A to tau protein with frontotemporal dementia and parkinsonism linked to chromosome 17 mutations
AU - Goedert, M.
AU - Satumtira, S.
AU - Jakes, R.
AU - Smith, M. J.
AU - Kamibayashi, C.
AU - White, C. L.
AU - Sontag, E.
PY - 2000
Y1 - 2000
N2 - Coding region and intronic mutations in the tau gene cause frontotemporal dementia and parkinsonism linked to chromosome 17 (FTDP-17). We have previously reported that ABαC, a major form of protein phosphatase 2A (PP2A) in brain, binds tightly to tau protein in vitro and is a major tau phosphatase in vivo. Using in vitro assays, we show here that the FTDP-17 mutations G272V, ΔK280, P301L, P301S, S305N, V337M, G389R, and R406W inhibit by˜20-95% the binding of recombinant three-repeat and four-repeat tau isoforms to the ABαC holoenzyme and the AC core enzyme of PP2A. Reduction in binding was maximal for tau proteins with the G272V, ΔK280, and V337M mutations. We also show that tau protein can be specifically coimmunoprecipitated with endogenous PP2A from both rat brain and transfected cell extracts. It is significant that, by using similar coimmunoprecipitation assays, we show that all FTDP-17 mutations tested, including the N279K mutation, alter the ability of tau to associate with cellular PP2A. Taken together, these results indicate that FTDP-17 mutations induce a significant decrease in the binding affinity of tau for PP2A in vivo. We propose that altered protein-protein interactions between PP2A and tau may contribute to FTDP-17 pathogenesis.
AB - Coding region and intronic mutations in the tau gene cause frontotemporal dementia and parkinsonism linked to chromosome 17 (FTDP-17). We have previously reported that ABαC, a major form of protein phosphatase 2A (PP2A) in brain, binds tightly to tau protein in vitro and is a major tau phosphatase in vivo. Using in vitro assays, we show here that the FTDP-17 mutations G272V, ΔK280, P301L, P301S, S305N, V337M, G389R, and R406W inhibit by˜20-95% the binding of recombinant three-repeat and four-repeat tau isoforms to the ABαC holoenzyme and the AC core enzyme of PP2A. Reduction in binding was maximal for tau proteins with the G272V, ΔK280, and V337M mutations. We also show that tau protein can be specifically coimmunoprecipitated with endogenous PP2A from both rat brain and transfected cell extracts. It is significant that, by using similar coimmunoprecipitation assays, we show that all FTDP-17 mutations tested, including the N279K mutation, alter the ability of tau to associate with cellular PP2A. Taken together, these results indicate that FTDP-17 mutations induce a significant decrease in the binding affinity of tau for PP2A in vivo. We propose that altered protein-protein interactions between PP2A and tau may contribute to FTDP-17 pathogenesis.
KW - Frontotemporal dementia
KW - Frontotemporal dementia and parkinsonism linked to chromosome 17
KW - Protein phosphatase 2A
KW - Tau mutations
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U2 - 10.1046/j.1471-4159.2000.0752155.x
DO - 10.1046/j.1471-4159.2000.0752155.x
M3 - Article
C2 - 11032905
AN - SCOPUS:0033788787
SN - 0022-3042
VL - 75
SP - 2155
EP - 2162
JO - Journal of Neurochemistry
JF - Journal of Neurochemistry
IS - 5
ER -