Reduced high-density lipoprotein 2b in non-obese type 2 diabetic patients analysed by a microfluidic chip method in a case-control study

Xunde Xian, Yahong Ma, Danhui D. Yang, Wei Huang, Yuhui Wang, Odilo Mueller, Elain Chang, Yves Konigshofer, Mark Van-Cleve, Jinkui Yang, George Liu

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

Background: Alterations in high-density lipoprotein (HDL) subfractions, especially in the HDL2b subfraction, have been reported in type 2 diabetes mellitus (T2DM). However, new methods for convenient and reliable quantitation of HDL2b are yet to be developed. Methods: Thirty-eight patients with T2DM were enrolled and age-, sex- and body mass index (BMI)-matched controls were selected from the same population. A microfluidic chip method was employed to analyse serum HDL subfractions. Results: The microfluidic chip method revealed a significant reduction in HDL2b and its ratio to total HDL in T2DM patients. There was a reverse correlation for total HDL and HDL2b, and its ratio with triglycerides, homeostasis model assessment-insulin sensitivity index (HOMA-IS) and insulin resistance index (HOMA-IR). Conclusions: We have shown a reduction of HDL2b and its ratio to total HDL by a novel chip method in T2DM patients. The significant correlation between HDL2b and HOMA-IS and HOMA-IR may have further predictive value in clinical utility.

Original languageEnglish (US)
Pages (from-to)619-623
Number of pages5
JournalBiomarkers
Volume14
Issue number8
DOIs
StatePublished - Dec 2009

Keywords

  • Bioanalyser
  • HDL subfractions
  • Microfluidic chip
  • Type 2 diabetes

ASJC Scopus subject areas

  • Biochemistry
  • Clinical Biochemistry
  • Health, Toxicology and Mutagenesis

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    Xian, X., Ma, Y., Yang, D. D., Huang, W., Wang, Y., Mueller, O., Chang, E., Konigshofer, Y., Van-Cleve, M., Yang, J., & Liu, G. (2009). Reduced high-density lipoprotein 2b in non-obese type 2 diabetic patients analysed by a microfluidic chip method in a case-control study. Biomarkers, 14(8), 619-623. https://doi.org/10.3109/13547500903204636