Reduced physeal area and chondrocyte proliferation in Pasteurella multocida toxin-treated rats

M. R. Ackermann, J. R. Stabel, R. K. Pettit, C. D. Jacobson, J. K. Elmquist, K. B. Register, R. B. Rimler, J. H. Hilton

Research output: Contribution to journalArticle

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Abstract

Pasteurella multocida toxin depresses weight gain in rats and pigs. It also affects tissues with rapidly dividing cells. In the present study, we investigated the role of this protein toxin on chondrocyte growth in vivo. Rats were divided into a single- or multiple-dose group and were given, respectively, either a single injection (0.15 or 0.6 μg/kg toxin subcutaneously) or multiple injections (0.01-0.2 μg/kg subcutaneously) of toxin. Bone (humerus) and other selected tissues were stained for bromodeoxyuridine immunoreactivity (BrDU-IR) in order to gauge cell proliferation. Physeal area was measured in rats from the multiple-dose group. Serum from single- and multiple-dose groups were tested for tumor necrosis factor α (TNF-α) and interleukin 6 (IL-6) activity using a bioassay system. Decreased weight gain, feed intake, and feed efficiency were observed in single- and multiple dose groups of rats. Decreased BrDU-IR indices were present in the resting and proliferative zone chondrocytes of the humeral physis in rats from the multiple-dose group, as was decreased physeal area. Increased serum IL-6 bioactivity was present in rats after 24 hours, and no changes in TNF-α bioactivity were seen in any group. No alterations in BrDU-IR were seen in rats fed restricted (80% of control) diets. These studies show that sublethal doses of toxin decrease weight gain and affect growth of long bones through suppression of chondrocyte proliferation. These effects may be mediated by direct binding of the toxin to target cells or IL-6 but are not associated with altered feed intake of TNF-induced cachexia.

Original languageEnglish (US)
Pages (from-to)674-682
Number of pages9
JournalVeterinary Pathology
Volume32
Issue number6
StatePublished - 1995

Fingerprint

chondrocytes
Pasteurella multocida
Chondrocytes
toxins
rats
tumor necrosis factors
Bromodeoxyuridine
dosage
interleukin-6
Weight Gain
Interleukin-6
Tumor Necrosis Factor-alpha
weight gain
feed intake
bones
injection
cachexia
Cachexia
Injections
humerus

Keywords

  • atrophic rhinitis
  • growth plate
  • immunohistochemistry

ASJC Scopus subject areas

  • veterinary(all)

Cite this

Ackermann, M. R., Stabel, J. R., Pettit, R. K., Jacobson, C. D., Elmquist, J. K., Register, K. B., ... Hilton, J. H. (1995). Reduced physeal area and chondrocyte proliferation in Pasteurella multocida toxin-treated rats. Veterinary Pathology, 32(6), 674-682.

Reduced physeal area and chondrocyte proliferation in Pasteurella multocida toxin-treated rats. / Ackermann, M. R.; Stabel, J. R.; Pettit, R. K.; Jacobson, C. D.; Elmquist, J. K.; Register, K. B.; Rimler, R. B.; Hilton, J. H.

In: Veterinary Pathology, Vol. 32, No. 6, 1995, p. 674-682.

Research output: Contribution to journalArticle

Ackermann, MR, Stabel, JR, Pettit, RK, Jacobson, CD, Elmquist, JK, Register, KB, Rimler, RB & Hilton, JH 1995, 'Reduced physeal area and chondrocyte proliferation in Pasteurella multocida toxin-treated rats', Veterinary Pathology, vol. 32, no. 6, pp. 674-682.
Ackermann MR, Stabel JR, Pettit RK, Jacobson CD, Elmquist JK, Register KB et al. Reduced physeal area and chondrocyte proliferation in Pasteurella multocida toxin-treated rats. Veterinary Pathology. 1995;32(6):674-682.
Ackermann, M. R. ; Stabel, J. R. ; Pettit, R. K. ; Jacobson, C. D. ; Elmquist, J. K. ; Register, K. B. ; Rimler, R. B. ; Hilton, J. H. / Reduced physeal area and chondrocyte proliferation in Pasteurella multocida toxin-treated rats. In: Veterinary Pathology. 1995 ; Vol. 32, No. 6. pp. 674-682.
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