Reducing susceptibility to bacteremia after experimental burn injury: A role for selective decontamination of the digestive tract

Jureta W. Horton, David L. Maass, Jean White, Joseph P. Minei

Research output: Contribution to journalArticlepeer-review

11 Scopus citations

Abstract

We proposed that selective decontamination of the digestive tract (SDD) initiated after experimental burn injury would decrease myocardial inflammation and dysfunction after a second insult such as septic challenge. Rats were divided into eight experimental groups. Groups included sham burn plus sham sepsis, burn alone, sepsis alone, and burn plus sepsis given either water by oral gavage for 5 days after burn (or sham burn) or given oral antibiotics (polymyxin E, 15 mg; tobramycin, 6 mg; 5-flucytosin, 100 mg given by oral gavage, 2X daily for 5 days after burn or sham burn). Cardiac function and inflammation were studied 24 h after septic challenge. In the absence of SDD, burn alone, sepsis alone, or burn plus septic challenge promoted cardiac myocyte secretion of TNF-α (burn, 174 ± 11; sepsis, 269 ± 19; burn ± sepsis, 453 ± 14 pg/ml), IL-1β (burn, 35 ± 2; sepsis, 29 ± 1; burn + sepsis, 48 ± 7 pg/ml), and IL-6 (burn, 143 ± 18; sepsis, 116 ± 3; burn + sepsis, 248 ± 12 pg/ml) compared with values measured in sham (TNF-α, 3 ± 1; IL-1β, 1 ± 0.4; IL-6, 6 ± 1.5 pg/ml) (P -< 0.05). Impaired ventricular contraction and relaxation responses were evident in the absence of SDD [burn + sepsis: left ventricular pressure (LVP), 65 ± 4 mmHg; rate of LVP rise (+dP/dt), 1,320 ± 131 mmHg/s compared with values measured in sham: LVP, 96 ± 4 mmHg; +dP/dt, 2,095 ± 99 mmHg/s, P -< 0.05]. SDD treatment of experimental burn attenuated septic challenge-related inflammatory responses and improved myocardial contractile responses, producing cardiac TNF-α, IL-1β, and IL-6 levels, LVP, +dP/dt, and rate of LVP fall (-dP/dt) values that were significantly better (P <- 0.05) than values measured in burn plus sepsis in the absence of SDD. This work confirms that endogenous gut organisms contribute to sensitivity to subsequent infectious challenge.

Original languageEnglish (US)
Pages (from-to)2207-2216
Number of pages10
JournalJournal of applied physiology
Volume102
Issue number6
DOIs
StatePublished - Jun 2007

Keywords

  • Adult Sprague-Dawley rats
  • Inflammatory cytokines
  • Intratracheal bacterial challenge
  • Isolated cardiac myocytes
  • Oral antibiotics
  • Postburn cardiac function
  • Streptococcus pneumoniae

ASJC Scopus subject areas

  • Physiology
  • Physiology (medical)

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