Reduction of BRCA1 expression in sporadic ovarian cancer

Wenxin Zheng, Feng Luo, Jean J. Lu, Armine Baltayan, Michael F. Press, Zuo Feng Zhang, Malcolm C. Pike

Research output: Contribution to journalArticle

48 Citations (Scopus)

Abstract

Objective. The purpose of this study was to examine BRCA1 expression and its relationship to cell proliferation in sporadic ovarian epithelial tumors (OETs). Methods. We investigated BRCA1 expression and cell proliferative activity in 72 unselected OETs. They comprised 16 benign cystadenomas, 18 borderline (low malignant potential) tumors, and 38 carcinomas (OECs). These patients had no known family history of breast and/or ovarian cancer. BRCA1 and the cell proliferation marker, MIB-1, expressions in fixed tissue were investigated in all 72 cases by immunohistochemistry (IHC). BRCA 1 mRNA in fresh frozen tissue samples from 20 of these cases was measured by a semi-quantitative reverse transcription- polymerase chain reaction (RT-PCR) method. Results. The average percentage of BRCA1-positive cells was 5.6% in cystadenomas, 29.7% in borderline tumors, and 6.6% in OECs. The average decreased steadily with increasing grade of OECs: grade 1 (21.4%), grade 2 (1.1%), and grade 3 (0%). The average percentage of MIB-1-positive cells increased steadily from cystadenomas (7.5%) to borderline tumors (22.6%) to carcinomas (41.2%). BRCA1 expression was highly correlated with MIB-1 expression in cystadenomas and borderline tumors. Six of seven OECs negative for BRCA1 by IHC showed low levels of BRCA1 mRNA by RT-PCR. Conclusions. BRCA1 expression paralleled cell proliferation in benign and borderline OETs, but not in OECs. Sporadic OECs showed significantly reduced levels, rather than complete loss, of BRCA1 expression. The reduction was closely related to tumor grade. Reduction of BRCA1 expression may be of etiologic significance in the occurrence and progression of sporadic ovarian cancer. (C) 2000 Academic Press.

Original languageEnglish (US)
Pages (from-to)294-300
Number of pages7
JournalGynecologic Oncology
Volume76
Issue number3
DOIs
StatePublished - Jan 1 2000

Fingerprint

Ovarian Neoplasms
Cystadenoma
Neoplasms
Cell Proliferation
Reverse Transcription
Immunohistochemistry
Carcinoma
Polymerase Chain Reaction
Messenger RNA
Breast Neoplasms

Keywords

  • BRCA1
  • Cell proliferation
  • Ovarian cancer

ASJC Scopus subject areas

  • Oncology
  • Obstetrics and Gynecology

Cite this

Zheng, W., Luo, F., Lu, J. J., Baltayan, A., Press, M. F., Zhang, Z. F., & Pike, M. C. (2000). Reduction of BRCA1 expression in sporadic ovarian cancer. Gynecologic Oncology, 76(3), 294-300. https://doi.org/10.1006/gyno.1999.5664

Reduction of BRCA1 expression in sporadic ovarian cancer. / Zheng, Wenxin; Luo, Feng; Lu, Jean J.; Baltayan, Armine; Press, Michael F.; Zhang, Zuo Feng; Pike, Malcolm C.

In: Gynecologic Oncology, Vol. 76, No. 3, 01.01.2000, p. 294-300.

Research output: Contribution to journalArticle

Zheng, W, Luo, F, Lu, JJ, Baltayan, A, Press, MF, Zhang, ZF & Pike, MC 2000, 'Reduction of BRCA1 expression in sporadic ovarian cancer', Gynecologic Oncology, vol. 76, no. 3, pp. 294-300. https://doi.org/10.1006/gyno.1999.5664
Zheng W, Luo F, Lu JJ, Baltayan A, Press MF, Zhang ZF et al. Reduction of BRCA1 expression in sporadic ovarian cancer. Gynecologic Oncology. 2000 Jan 1;76(3):294-300. https://doi.org/10.1006/gyno.1999.5664
Zheng, Wenxin ; Luo, Feng ; Lu, Jean J. ; Baltayan, Armine ; Press, Michael F. ; Zhang, Zuo Feng ; Pike, Malcolm C. / Reduction of BRCA1 expression in sporadic ovarian cancer. In: Gynecologic Oncology. 2000 ; Vol. 76, No. 3. pp. 294-300.
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abstract = "Objective. The purpose of this study was to examine BRCA1 expression and its relationship to cell proliferation in sporadic ovarian epithelial tumors (OETs). Methods. We investigated BRCA1 expression and cell proliferative activity in 72 unselected OETs. They comprised 16 benign cystadenomas, 18 borderline (low malignant potential) tumors, and 38 carcinomas (OECs). These patients had no known family history of breast and/or ovarian cancer. BRCA1 and the cell proliferation marker, MIB-1, expressions in fixed tissue were investigated in all 72 cases by immunohistochemistry (IHC). BRCA 1 mRNA in fresh frozen tissue samples from 20 of these cases was measured by a semi-quantitative reverse transcription- polymerase chain reaction (RT-PCR) method. Results. The average percentage of BRCA1-positive cells was 5.6{\%} in cystadenomas, 29.7{\%} in borderline tumors, and 6.6{\%} in OECs. The average decreased steadily with increasing grade of OECs: grade 1 (21.4{\%}), grade 2 (1.1{\%}), and grade 3 (0{\%}). The average percentage of MIB-1-positive cells increased steadily from cystadenomas (7.5{\%}) to borderline tumors (22.6{\%}) to carcinomas (41.2{\%}). BRCA1 expression was highly correlated with MIB-1 expression in cystadenomas and borderline tumors. Six of seven OECs negative for BRCA1 by IHC showed low levels of BRCA1 mRNA by RT-PCR. Conclusions. BRCA1 expression paralleled cell proliferation in benign and borderline OETs, but not in OECs. Sporadic OECs showed significantly reduced levels, rather than complete loss, of BRCA1 expression. The reduction was closely related to tumor grade. Reduction of BRCA1 expression may be of etiologic significance in the occurrence and progression of sporadic ovarian cancer. (C) 2000 Academic Press.",
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N2 - Objective. The purpose of this study was to examine BRCA1 expression and its relationship to cell proliferation in sporadic ovarian epithelial tumors (OETs). Methods. We investigated BRCA1 expression and cell proliferative activity in 72 unselected OETs. They comprised 16 benign cystadenomas, 18 borderline (low malignant potential) tumors, and 38 carcinomas (OECs). These patients had no known family history of breast and/or ovarian cancer. BRCA1 and the cell proliferation marker, MIB-1, expressions in fixed tissue were investigated in all 72 cases by immunohistochemistry (IHC). BRCA 1 mRNA in fresh frozen tissue samples from 20 of these cases was measured by a semi-quantitative reverse transcription- polymerase chain reaction (RT-PCR) method. Results. The average percentage of BRCA1-positive cells was 5.6% in cystadenomas, 29.7% in borderline tumors, and 6.6% in OECs. The average decreased steadily with increasing grade of OECs: grade 1 (21.4%), grade 2 (1.1%), and grade 3 (0%). The average percentage of MIB-1-positive cells increased steadily from cystadenomas (7.5%) to borderline tumors (22.6%) to carcinomas (41.2%). BRCA1 expression was highly correlated with MIB-1 expression in cystadenomas and borderline tumors. Six of seven OECs negative for BRCA1 by IHC showed low levels of BRCA1 mRNA by RT-PCR. Conclusions. BRCA1 expression paralleled cell proliferation in benign and borderline OETs, but not in OECs. Sporadic OECs showed significantly reduced levels, rather than complete loss, of BRCA1 expression. The reduction was closely related to tumor grade. Reduction of BRCA1 expression may be of etiologic significance in the occurrence and progression of sporadic ovarian cancer. (C) 2000 Academic Press.

AB - Objective. The purpose of this study was to examine BRCA1 expression and its relationship to cell proliferation in sporadic ovarian epithelial tumors (OETs). Methods. We investigated BRCA1 expression and cell proliferative activity in 72 unselected OETs. They comprised 16 benign cystadenomas, 18 borderline (low malignant potential) tumors, and 38 carcinomas (OECs). These patients had no known family history of breast and/or ovarian cancer. BRCA1 and the cell proliferation marker, MIB-1, expressions in fixed tissue were investigated in all 72 cases by immunohistochemistry (IHC). BRCA 1 mRNA in fresh frozen tissue samples from 20 of these cases was measured by a semi-quantitative reverse transcription- polymerase chain reaction (RT-PCR) method. Results. The average percentage of BRCA1-positive cells was 5.6% in cystadenomas, 29.7% in borderline tumors, and 6.6% in OECs. The average decreased steadily with increasing grade of OECs: grade 1 (21.4%), grade 2 (1.1%), and grade 3 (0%). The average percentage of MIB-1-positive cells increased steadily from cystadenomas (7.5%) to borderline tumors (22.6%) to carcinomas (41.2%). BRCA1 expression was highly correlated with MIB-1 expression in cystadenomas and borderline tumors. Six of seven OECs negative for BRCA1 by IHC showed low levels of BRCA1 mRNA by RT-PCR. Conclusions. BRCA1 expression paralleled cell proliferation in benign and borderline OETs, but not in OECs. Sporadic OECs showed significantly reduced levels, rather than complete loss, of BRCA1 expression. The reduction was closely related to tumor grade. Reduction of BRCA1 expression may be of etiologic significance in the occurrence and progression of sporadic ovarian cancer. (C) 2000 Academic Press.

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