Reductions in platelet contractile force correlate with duration of cardiopulmonary bypass and blood loss in patients undergoing cardiac surgery

Philip E Greilich, Chad F. Brouse, Joseph Beckham, Michael E Jessen, Erika J. Martin, Marcus E. Carr

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Blood loss secondary to platelet dysfunction is known to be increased when the duration of cardiopulmonary bypass (CPB) is prolonged. The ability to correlate alterations in platelet function with the duration of bypass and early postoperative blood loss, however, has remained elusive. Platelet contractile force, a novel measure of platelet-mediated clot retraction, is known to be reduced following cardiac surgery and blockade of platelet adhesion receptors. The aim of this study was to determine if alterations in platelet contractile force (measured using whole blood) correlated with the duration of CPB and early postoperative blood loss. Thirty patients were entered into a study designed to measure platelet function before, during, and after CPB. Platelet aggregometry and surface expression of CD42b and CD61 were also measured (using whole blood) in a subset of subjects (n=10) to further characterize the intrinsic structural and functional defects induced by CPB. Reductions in platelet contractile force had a significant correlation with duration of CPB (r=0.564; P=0.002) and early blood loss (r=0.545; P=0.003). Although decreases in platelet contractile force and aggregation both correlated with CPB time in the smaller subset of patients tested, only platelet contractile force correlated with decreases in CD42b, CD61 and blood loss. The results of this study suggest that prolongation of CPB is related to increasing degrees of platelet dysfunction and that reductions in platelet contractile force are related to decreases in platelet adhesion receptors and early postoperative blood loss.

Original languageEnglish (US)
Pages (from-to)523-529
Number of pages7
JournalThrombosis Research
Volume105
Issue number6
DOIs
StatePublished - Mar 15 2002

Fingerprint

Cardiopulmonary Bypass
Thoracic Surgery
Blood Platelets
Postoperative Hemorrhage
Clot Retraction

Keywords

  • Cardiopulmonary bypass
  • Hemorrhage
  • Platelet adhesion receptors
  • Platelet aggregation
  • Platelet contractile force

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine
  • Hematology

Cite this

Reductions in platelet contractile force correlate with duration of cardiopulmonary bypass and blood loss in patients undergoing cardiac surgery. / Greilich, Philip E; Brouse, Chad F.; Beckham, Joseph; Jessen, Michael E; Martin, Erika J.; Carr, Marcus E.

In: Thrombosis Research, Vol. 105, No. 6, 15.03.2002, p. 523-529.

Research output: Contribution to journalArticle

@article{d43d180ed7f3465c9f06be1fe3135fe3,
title = "Reductions in platelet contractile force correlate with duration of cardiopulmonary bypass and blood loss in patients undergoing cardiac surgery",
abstract = "Blood loss secondary to platelet dysfunction is known to be increased when the duration of cardiopulmonary bypass (CPB) is prolonged. The ability to correlate alterations in platelet function with the duration of bypass and early postoperative blood loss, however, has remained elusive. Platelet contractile force, a novel measure of platelet-mediated clot retraction, is known to be reduced following cardiac surgery and blockade of platelet adhesion receptors. The aim of this study was to determine if alterations in platelet contractile force (measured using whole blood) correlated with the duration of CPB and early postoperative blood loss. Thirty patients were entered into a study designed to measure platelet function before, during, and after CPB. Platelet aggregometry and surface expression of CD42b and CD61 were also measured (using whole blood) in a subset of subjects (n=10) to further characterize the intrinsic structural and functional defects induced by CPB. Reductions in platelet contractile force had a significant correlation with duration of CPB (r=0.564; P=0.002) and early blood loss (r=0.545; P=0.003). Although decreases in platelet contractile force and aggregation both correlated with CPB time in the smaller subset of patients tested, only platelet contractile force correlated with decreases in CD42b, CD61 and blood loss. The results of this study suggest that prolongation of CPB is related to increasing degrees of platelet dysfunction and that reductions in platelet contractile force are related to decreases in platelet adhesion receptors and early postoperative blood loss.",
keywords = "Cardiopulmonary bypass, Hemorrhage, Platelet adhesion receptors, Platelet aggregation, Platelet contractile force",
author = "Greilich, {Philip E} and Brouse, {Chad F.} and Joseph Beckham and Jessen, {Michael E} and Martin, {Erika J.} and Carr, {Marcus E.}",
year = "2002",
month = "3",
day = "15",
doi = "10.1016/S0049-3848(02)00061-0",
language = "English (US)",
volume = "105",
pages = "523--529",
journal = "Thrombosis Research",
issn = "0049-3848",
publisher = "Elsevier Limited",
number = "6",

}

TY - JOUR

T1 - Reductions in platelet contractile force correlate with duration of cardiopulmonary bypass and blood loss in patients undergoing cardiac surgery

AU - Greilich, Philip E

AU - Brouse, Chad F.

AU - Beckham, Joseph

AU - Jessen, Michael E

AU - Martin, Erika J.

AU - Carr, Marcus E.

PY - 2002/3/15

Y1 - 2002/3/15

N2 - Blood loss secondary to platelet dysfunction is known to be increased when the duration of cardiopulmonary bypass (CPB) is prolonged. The ability to correlate alterations in platelet function with the duration of bypass and early postoperative blood loss, however, has remained elusive. Platelet contractile force, a novel measure of platelet-mediated clot retraction, is known to be reduced following cardiac surgery and blockade of platelet adhesion receptors. The aim of this study was to determine if alterations in platelet contractile force (measured using whole blood) correlated with the duration of CPB and early postoperative blood loss. Thirty patients were entered into a study designed to measure platelet function before, during, and after CPB. Platelet aggregometry and surface expression of CD42b and CD61 were also measured (using whole blood) in a subset of subjects (n=10) to further characterize the intrinsic structural and functional defects induced by CPB. Reductions in platelet contractile force had a significant correlation with duration of CPB (r=0.564; P=0.002) and early blood loss (r=0.545; P=0.003). Although decreases in platelet contractile force and aggregation both correlated with CPB time in the smaller subset of patients tested, only platelet contractile force correlated with decreases in CD42b, CD61 and blood loss. The results of this study suggest that prolongation of CPB is related to increasing degrees of platelet dysfunction and that reductions in platelet contractile force are related to decreases in platelet adhesion receptors and early postoperative blood loss.

AB - Blood loss secondary to platelet dysfunction is known to be increased when the duration of cardiopulmonary bypass (CPB) is prolonged. The ability to correlate alterations in platelet function with the duration of bypass and early postoperative blood loss, however, has remained elusive. Platelet contractile force, a novel measure of platelet-mediated clot retraction, is known to be reduced following cardiac surgery and blockade of platelet adhesion receptors. The aim of this study was to determine if alterations in platelet contractile force (measured using whole blood) correlated with the duration of CPB and early postoperative blood loss. Thirty patients were entered into a study designed to measure platelet function before, during, and after CPB. Platelet aggregometry and surface expression of CD42b and CD61 were also measured (using whole blood) in a subset of subjects (n=10) to further characterize the intrinsic structural and functional defects induced by CPB. Reductions in platelet contractile force had a significant correlation with duration of CPB (r=0.564; P=0.002) and early blood loss (r=0.545; P=0.003). Although decreases in platelet contractile force and aggregation both correlated with CPB time in the smaller subset of patients tested, only platelet contractile force correlated with decreases in CD42b, CD61 and blood loss. The results of this study suggest that prolongation of CPB is related to increasing degrees of platelet dysfunction and that reductions in platelet contractile force are related to decreases in platelet adhesion receptors and early postoperative blood loss.

KW - Cardiopulmonary bypass

KW - Hemorrhage

KW - Platelet adhesion receptors

KW - Platelet aggregation

KW - Platelet contractile force

UR - http://www.scopus.com/inward/record.url?scp=0037085704&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0037085704&partnerID=8YFLogxK

U2 - 10.1016/S0049-3848(02)00061-0

DO - 10.1016/S0049-3848(02)00061-0

M3 - Article

VL - 105

SP - 523

EP - 529

JO - Thrombosis Research

JF - Thrombosis Research

SN - 0049-3848

IS - 6

ER -