Redundant roles of SMAD2 and SMAD3 in ovarian granulosa cells in vivo

Qinglei Li, Stephanie A. Pangas, Carolina J. Jorgez, Jonathan M. Graff, Michael Weinstein, Martin M. Matzuk

Research output: Contribution to journalArticle

105 Citations (Scopus)

Abstract

Transforming growth factor β (TGF-β) superfamily members are critical in maintaining cell growth and differentiation in the ovary. Although signaling of activins, TGF-βs, growth differentiation factor 9, and nodal converge preferentially to SMAD2 and SMAD3, the in vivo functions and redundancy of these SMADs in the ovary and female reproduction remain largely unidentified. To circumvent the deleterious phenotypic aspects of ubiquitous deletion of Smad2 and Smad3, a conditional knockout strategy was formulated to selectively inactivate Smad2, Smad3, or both Smad2 and Smad3 in ovarian granulosa cells. While granulosa cell ablation of individual Smad2 or Smad3 caused insignificant changes in female fertility, deletion of both Smad2 and Smad3 led to dramatically reduced female fertility and fecundity. These defects were associated with the disruption of multiple ovarian processes, including follicular development, ovulation, and cumulus cell expansion. Furthermore, the impaired expansion of cumulus cells may be partially associated with altered cumulus expansion-related transcripts that are regulated by SMAD2/3 signaling. Our results indicate that SMAD2 and SMAD3 function redundantly in vivo to maintain normal female fertility and further support the involvement of an intraovarian SMAD2/3 pathway in mediating oocyte-produced signals essential for coordinating key events of the ovulatory process.

Original languageEnglish (US)
Pages (from-to)7001-7011
Number of pages11
JournalMolecular and Cellular Biology
Volume28
Issue number23
DOIs
StatePublished - Dec 2008

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Granulosa Cells
Fertility
Cumulus Cells
Transforming Growth Factors
Ovary
Growth Differentiation Factor 9
Activins
Ovulation
Oocytes
Reproduction
Cell Differentiation
Growth

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

Cite this

Li, Q., Pangas, S. A., Jorgez, C. J., Graff, J. M., Weinstein, M., & Matzuk, M. M. (2008). Redundant roles of SMAD2 and SMAD3 in ovarian granulosa cells in vivo. Molecular and Cellular Biology, 28(23), 7001-7011. https://doi.org/10.1128/MCB.00732-08

Redundant roles of SMAD2 and SMAD3 in ovarian granulosa cells in vivo. / Li, Qinglei; Pangas, Stephanie A.; Jorgez, Carolina J.; Graff, Jonathan M.; Weinstein, Michael; Matzuk, Martin M.

In: Molecular and Cellular Biology, Vol. 28, No. 23, 12.2008, p. 7001-7011.

Research output: Contribution to journalArticle

Li, Q, Pangas, SA, Jorgez, CJ, Graff, JM, Weinstein, M & Matzuk, MM 2008, 'Redundant roles of SMAD2 and SMAD3 in ovarian granulosa cells in vivo', Molecular and Cellular Biology, vol. 28, no. 23, pp. 7001-7011. https://doi.org/10.1128/MCB.00732-08
Li, Qinglei ; Pangas, Stephanie A. ; Jorgez, Carolina J. ; Graff, Jonathan M. ; Weinstein, Michael ; Matzuk, Martin M. / Redundant roles of SMAD2 and SMAD3 in ovarian granulosa cells in vivo. In: Molecular and Cellular Biology. 2008 ; Vol. 28, No. 23. pp. 7001-7011.
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