Reelin Mobilizes a VAMP7-Dependent Synaptic Vesicle Pool and Selectively Augments Spontaneous Neurotransmission

Manjot Bal, Jeremy Leitz, Austin L. Reese, Denise M O Ramirez, Murat S Durakoglugil, Joachim Herz, Lisa M Monteggia, Ege T Kavalali

Research output: Contribution to journalArticlepeer-review

75 Scopus citations

Abstract

Reelin is a glycoprotein that is critical for proper layering of neocortex during development as well as dynamic regulation of glutamatergic postsynaptic signaling in mature synapses. Here, we show that Reelin also acts presynaptically, resulting in robust rapid enhancement of spontaneous neurotransmitter release without affecting properties of evoked neurotransmission. This effect of Reelin requires a modest but significant increase in presynaptic Ca2+ initiatedvia ApoER2 signaling. The specificity of Reelin actionon spontaneous neurotransmitter release is encodedat the level of vesicular SNARE machinery as it requires VAMP7 and SNAP-25 but not synaptobrevin2, VAMP4, or vti1a. These results uncover a presynaptic regulatory pathway that utilizes the heterogeneity ofsynaptic vesicle-associated SNAREs and selectively augments action potential-independent neurotransmission

Original languageEnglish (US)
Pages (from-to)934-946
Number of pages13
JournalNeuron
Volume80
Issue number4
DOIs
StatePublished - Nov 20 2013

ASJC Scopus subject areas

  • Neuroscience(all)

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