Reelin signaling directly affects radial glia morphology and biochemical maturation

Eva Hartfuss, Eckart Förster, Hans H. Bock, Michael A. Hack, Pierre Leprince, Juan M. Lugue, Joachim Herz, Michael Frotscher, Magdalena Götz

Research output: Contribution to journalArticle

173 Citations (Scopus)

Abstract

Radial glial cells are characterized, besides their astroglial properties, by long radial processes extending from the ventricular zone to the pial surface, a crucial feature for the radial migration of neurons. The molecular signals that regulate this characteristic morphology, however, are largely unknown. We show an important role of the secreted molecule reelin for the establishment of radial glia processes. We describe a significant reduction in ventricular zone cells with long radial processes in the absence of reelin in the cortex of reeler mutant mice. These defects were correlated to a decrease in the content of brain lipid-binding protein (Blbp) and were detected exclusively in the cerebral cortex, but not in the basal ganglia of reeler mice. Conversely, reelin addition in vitro increased the Blbp content and process extension of radial glia from the cortex, but not the basal ganglia. Isolation of radial glia by fluorescent-activated cell sorting showed that these effects are due to direct signaling of reelin to radial glial cells. We could further demonstrate that this signaling requires Dab1, as the increase in Blbp upon reelin addition failed to occur in Dab1-/- mice. Taken together, these results unravel a novel role of reelin signaling to radial glial cells that is crucial for the regulation of their Blbp content and characteristic morphology in a region-specific manner.

Original languageEnglish (US)
Pages (from-to)4597-4609
Number of pages13
JournalDevelopment
Volume130
Issue number19
DOIs
StatePublished - Oct 2003

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Ependymoglial Cells
Neuroglia
Neurologic Mutant Mice
Basal Ganglia
Cerebral Cortex
Neurons
Fatty Acid-Binding Protein 7

Keywords

  • Apoer2
  • Dab1
  • Neurogenesis
  • Precursor morphology
  • Reeler mutant
  • Vldlr

ASJC Scopus subject areas

  • Anatomy
  • Cell Biology

Cite this

Hartfuss, E., Förster, E., Bock, H. H., Hack, M. A., Leprince, P., Lugue, J. M., ... Götz, M. (2003). Reelin signaling directly affects radial glia morphology and biochemical maturation. Development, 130(19), 4597-4609. https://doi.org/10.1242/dev.00654

Reelin signaling directly affects radial glia morphology and biochemical maturation. / Hartfuss, Eva; Förster, Eckart; Bock, Hans H.; Hack, Michael A.; Leprince, Pierre; Lugue, Juan M.; Herz, Joachim; Frotscher, Michael; Götz, Magdalena.

In: Development, Vol. 130, No. 19, 10.2003, p. 4597-4609.

Research output: Contribution to journalArticle

Hartfuss, E, Förster, E, Bock, HH, Hack, MA, Leprince, P, Lugue, JM, Herz, J, Frotscher, M & Götz, M 2003, 'Reelin signaling directly affects radial glia morphology and biochemical maturation', Development, vol. 130, no. 19, pp. 4597-4609. https://doi.org/10.1242/dev.00654
Hartfuss E, Förster E, Bock HH, Hack MA, Leprince P, Lugue JM et al. Reelin signaling directly affects radial glia morphology and biochemical maturation. Development. 2003 Oct;130(19):4597-4609. https://doi.org/10.1242/dev.00654
Hartfuss, Eva ; Förster, Eckart ; Bock, Hans H. ; Hack, Michael A. ; Leprince, Pierre ; Lugue, Juan M. ; Herz, Joachim ; Frotscher, Michael ; Götz, Magdalena. / Reelin signaling directly affects radial glia morphology and biochemical maturation. In: Development. 2003 ; Vol. 130, No. 19. pp. 4597-4609.
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