Refined mapping of two regions of loss of heterozygosity on chromosome band 11q23 in lung cancer

Steven Siqing Wang, Arvind Virmani, Adi F. Gazdar, John D. Minna, Glen A. Evans

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

11q23-24 chromosome is a region containing frequent allelic loss (loss of heterozygosity; LOH) in human cancers. To examine cancer-related allelic loss in the region between D11S940 and APOC3, we used 17 polymorphic markers and allotyped 28 lung cancer-derived cell lines and their corresponding matched lymphoblastoid celt lines. LOH was found in 71.4% (20/28) of the lung cancer cell lines and was localized to two distinct minimal regions of loss. One region is bracketed by markers D11S1647 and NCAM2 and contains the gene encoding the β isoform of the A subunit of the human protein phosphatase 2A (PPP2R1B). Recently, mutations in this gene were described in lung and colon cancers, suggesting that PPP2R1B functions as a tumor-suppressor gene. A second minimal region of loss was defined between markers D11S1792 and D11S1885, a region estimated to be less than 1 Mb. Thus, chromosome 11 likely harbors two sites of suppressor oncogene activity in lung cancer, one defined by the PPP2R1B gene and the second located telomeric to PPP2R1B. This study facilitates the identification and cloning of a second critical tumor- suppressor gene involved in lung cancer, and possibly a variety of other cancers, on human chromosome band 11q23.

Original languageEnglish (US)
Pages (from-to)154-159
Number of pages6
JournalGenes Chromosomes and Cancer
Volume25
Issue number2
DOIs
StatePublished - Jun 1999

Fingerprint

Loss of Heterozygosity
Lung Neoplasms
Chromosomes
Tumor Suppressor Genes
Genes
Protein Phosphatase 2
Cell Line
Neoplasms
Chromosomes, Human, Pair 11
Human Chromosomes
Oncogenes
Colonic Neoplasms
Organism Cloning
Protein Isoforms
Mutation

ASJC Scopus subject areas

  • Cancer Research
  • Genetics

Cite this

Refined mapping of two regions of loss of heterozygosity on chromosome band 11q23 in lung cancer. / Wang, Steven Siqing; Virmani, Arvind; Gazdar, Adi F.; Minna, John D.; Evans, Glen A.

In: Genes Chromosomes and Cancer, Vol. 25, No. 2, 06.1999, p. 154-159.

Research output: Contribution to journalArticle

Wang, Steven Siqing ; Virmani, Arvind ; Gazdar, Adi F. ; Minna, John D. ; Evans, Glen A. / Refined mapping of two regions of loss of heterozygosity on chromosome band 11q23 in lung cancer. In: Genes Chromosomes and Cancer. 1999 ; Vol. 25, No. 2. pp. 154-159.
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