Regional and subcellular localization of luteinizing hormone releasing hormone in the adult human brain

C. Richard Parker, William B. Neaves, John C. Porter

Research output: Contribution to journalArticle

10 Scopus citations

Abstract

The localization of luteinizing hormone releasing hormone (LHRH) in the post mortem adult human brain was investigated. LHRH was highly concentrated in medial basal hypothalamic tissue (1.14 ng/mg protein); lower levels of LHRH were present in tissue from the optic chiasm (0.05 ng/mg protein) and mammillary bodies (0.07 ng/mg protein). The concentrations of LHRH in hypothalamic tissue of men and women were similar. LHRH was undetectable (<0.001 ng/mg protein) in the frontal cerebral cortex and cerebellum. When homogenates of the medial basal hypothalamus were fractionated on continuous or discontinuous sucrose density gradients, LHRH was found to be associated with subcellular particles. Upon examination by transmission electron microscopy, we found that these subcellular particles resembled isolated neuron terminals, i.e., synaptosomes. Low to undetectable amounts of LHRH were found in the cytosol or the myelin+microsome fraction of the gradients. The results of these studies are supportive of the view that LHRH is highly concentrated in neuron terminals of the adult human brain and may, therefore, be a central neurotransmitter or neuromodulator in the human.

Original languageEnglish (US)
Pages (from-to)307-313
Number of pages7
JournalBrain Research Bulletin
Volume5
Issue number3
DOIs
Publication statusPublished - 1980

    Fingerprint

Keywords

  • Human brain
  • Hypothalamus
  • LHRH
  • Luteinizing hormone releasing hormone
  • Neuronal peptide
  • Neurotransmitters
  • Subcellular fractionation
  • Subcellular morphology
  • Synaptosomes

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this