TY - JOUR
T1 - Regional assignment of a human dna repair gene (xrcc5) to 2q35 by x-ray hybrid mapping
AU - Chen, David J.
AU - Marrone, Babetta L.
AU - Nguyen, Thu
AU - Stackhouse, Murray
AU - Zhao, Ying
AU - Siciliano, Michael J.
PY - 1994/5/15
Y1 - 1994/5/15
N2 - A number of human genes involved in the repair of radiation-induced DNA double-strand breaks have been identified based on the analysis of radiation-sensitive rodent mutants. Recently, one of the DSB repair genes, XRCC5, has been mapped on human chromosome 2 by concordance analysis of somatic cell hybrids and by the microcell-mediated chromosome transfer approach. For the regional mapping of the human XRCC5 gene, we have constructed a panel of X-ray hybrids and a panel of microcell-mediated chromosome 2 hybrids in the mutant background that contain only fragments of human chromosome 2. By using fluorescence in situ hybridization, chromosome banding, and physical mapping of these X-ray hybrids, we have localized the human XRCC5 locus to 2q35. This result is further confirmed by a segregation analysis indicating that the radiation resistant phenotype of a repair-proficient hybrid cosegregates with the human 2q35 chromosome fragment.
AB - A number of human genes involved in the repair of radiation-induced DNA double-strand breaks have been identified based on the analysis of radiation-sensitive rodent mutants. Recently, one of the DSB repair genes, XRCC5, has been mapped on human chromosome 2 by concordance analysis of somatic cell hybrids and by the microcell-mediated chromosome transfer approach. For the regional mapping of the human XRCC5 gene, we have constructed a panel of X-ray hybrids and a panel of microcell-mediated chromosome 2 hybrids in the mutant background that contain only fragments of human chromosome 2. By using fluorescence in situ hybridization, chromosome banding, and physical mapping of these X-ray hybrids, we have localized the human XRCC5 locus to 2q35. This result is further confirmed by a segregation analysis indicating that the radiation resistant phenotype of a repair-proficient hybrid cosegregates with the human 2q35 chromosome fragment.
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U2 - 10.1006/geno.1994.1287
DO - 10.1006/geno.1994.1287
M3 - Article
C2 - 8088837
AN - SCOPUS:0028337066
SN - 0888-7543
VL - 21
SP - 423
EP - 427
JO - Genomics
JF - Genomics
IS - 2
ER -