Regional assignment of a human dna repair gene (xrcc5) to 2q35 by x-ray hybrid mapping

David J. Chen, Babetta L. Marrone, Thu Nguyen, Murray Stackhouse, Ying Zhao, Michael J. Siciliano

Research output: Contribution to journalArticle

23 Scopus citations

Abstract

A number of human genes involved in the repair of radiation-induced DNA double-strand breaks have been identified based on the analysis of radiation-sensitive rodent mutants. Recently, one of the DSB repair genes, XRCC5, has been mapped on human chromosome 2 by concordance analysis of somatic cell hybrids and by the microcell-mediated chromosome transfer approach. For the regional mapping of the human XRCC5 gene, we have constructed a panel of X-ray hybrids and a panel of microcell-mediated chromosome 2 hybrids in the mutant background that contain only fragments of human chromosome 2. By using fluorescence in situ hybridization, chromosome banding, and physical mapping of these X-ray hybrids, we have localized the human XRCC5 locus to 2q35. This result is further confirmed by a segregation analysis indicating that the radiation resistant phenotype of a repair-proficient hybrid cosegregates with the human 2q35 chromosome fragment.

Original languageEnglish (US)
Pages (from-to)423-427
Number of pages5
JournalGenomics
Volume21
Issue number2
DOIs
StatePublished - May 15 1994

ASJC Scopus subject areas

  • Genetics

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    Chen, D. J., Marrone, B. L., Nguyen, T., Stackhouse, M., Zhao, Y., & Siciliano, M. J. (1994). Regional assignment of a human dna repair gene (xrcc5) to 2q35 by x-ray hybrid mapping. Genomics, 21(2), 423-427. https://doi.org/10.1006/geno.1994.1287