Regional Assignment of a Human DNA Repair Gene (XRCC5) to 2q35 by X-Ray Hybrid Mapping

David J. Chen, Babetta L. Marrone, Thu Nguyen, Murray Stackhouse, Ying Zhao, Michael J. Siciliano

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

A number of human genes involved in the repair of radiation-induced DNA double-strand breaks have been identified based on the analysis of radiation-sensitive rodent mutants. Recently, one of the DSB repair genes, XRCC5, has been mapped on human chromosome 2 by concordance analysis of somatic cell hybrids and by the microcell-mediated chromosome transfer approach. For the regional mapping of the human XRCC5 gene, we have constructed a panel of X-ray hybrids and a panel of microcell-mediated chromosome 2 hybrids in the mutant background that contain only fragments of human chromosome 2. By using fluorescence in situ hybridization, chromosome banding, and physical mapping of these X-ray hybrids, we have localized the human XRCC5 locus to 2q35. This result is further confirmed by a segregation analysis indicating that the radiation resistant phenotype of a repair-proficient hybrid cosegregates with the human 2q35 chromosome fragment.

Original languageEnglish (US)
Pages (from-to)423-427
Number of pages5
JournalGenomics
Volume21
Issue number2
DOIs
StatePublished - May 15 1994

Fingerprint

DNA Repair
Chromosomes, Human, Pair 2
Human Chromosomes
X-Rays
Radiation
Genes
Chromosome Banding
Double-Stranded DNA Breaks
Hybrid Cells
Fluorescence In Situ Hybridization
Rodentia
Chromosomes
Phenotype

ASJC Scopus subject areas

  • Genetics

Cite this

Chen, D. J., Marrone, B. L., Nguyen, T., Stackhouse, M., Zhao, Y., & Siciliano, M. J. (1994). Regional Assignment of a Human DNA Repair Gene (XRCC5) to 2q35 by X-Ray Hybrid Mapping. Genomics, 21(2), 423-427. https://doi.org/10.1006/geno.1994.1287

Regional Assignment of a Human DNA Repair Gene (XRCC5) to 2q35 by X-Ray Hybrid Mapping. / Chen, David J.; Marrone, Babetta L.; Nguyen, Thu; Stackhouse, Murray; Zhao, Ying; Siciliano, Michael J.

In: Genomics, Vol. 21, No. 2, 15.05.1994, p. 423-427.

Research output: Contribution to journalArticle

Chen, DJ, Marrone, BL, Nguyen, T, Stackhouse, M, Zhao, Y & Siciliano, MJ 1994, 'Regional Assignment of a Human DNA Repair Gene (XRCC5) to 2q35 by X-Ray Hybrid Mapping', Genomics, vol. 21, no. 2, pp. 423-427. https://doi.org/10.1006/geno.1994.1287
Chen DJ, Marrone BL, Nguyen T, Stackhouse M, Zhao Y, Siciliano MJ. Regional Assignment of a Human DNA Repair Gene (XRCC5) to 2q35 by X-Ray Hybrid Mapping. Genomics. 1994 May 15;21(2):423-427. https://doi.org/10.1006/geno.1994.1287
Chen, David J. ; Marrone, Babetta L. ; Nguyen, Thu ; Stackhouse, Murray ; Zhao, Ying ; Siciliano, Michael J. / Regional Assignment of a Human DNA Repair Gene (XRCC5) to 2q35 by X-Ray Hybrid Mapping. In: Genomics. 1994 ; Vol. 21, No. 2. pp. 423-427.
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