Regional, cellular, and subcellular localization of RGS10 in rodent brain

Jeff L Waugh, Angela C. Lou, Amelia J Eisch, Lisa M Monteggia, E. Chris Muly, Stephen J. Gold

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

The regulator of G protein signaling type 10 (RGS10) modulates Gαi/o signaling by means of its GTPase accelerating activity and is abundantly expressed in brain and in immune tissues. To elucidate RGS10 function in the nervous system, we mapped RGS10 protein in rat and mouse brain using light microscopic (LM) and electron microscopic (EM) immunohistochemical techniques. The LM showed that RGS10-like immunoreactivity (LIR) labels all cellular subcompartments of neurons and microglia, including their nuclei. There were several differences between RGS10-LIR distributions in rat and mouse, the most striking of which were the far denser immunoreactivity in rat dentate gyrus and dorsal raphe. The EM analysis corroborated and extended our findings from LM. Thus, EM confirmed the presence of dense RGS10-LIR in the euchromatin compartment of nuclei. The EM analysis also resolved dense staining on terminals at symmetric synapses onto pyramidal cell somata. Dual immunofluorescence showed that forebrain interneurons densely labeled with RGS10-LIR partially colocalized with parvalbumin-LIR. Dual-labeling histochemistry in caudoputamen demonstrated that densely labeled striatal cells were biased to the indirect-projecting output pathway. Dual-labeling immunofluorescence also showed that densely labeled RGS10-LIR cells in the dentate gyrus subgranular zone were not proliferating but that newly born cells could differentiate to express RGS10-LIR. Taken together, these data support a role for RGS10 in diverse processes that include modulation of pre- and postsynaptic G-protein signaling. Moreover, enrichment of RGS10 in transcriptionally active regions of the nucleus suggests an unforeseen role of RGS10 in modulating gene expression.

Original languageEnglish (US)
Pages (from-to)299-313
Number of pages15
JournalJournal of Comparative Neurology
Volume481
Issue number3
DOIs
StatePublished - Jan 17 2005

Fingerprint

GTP-Binding Protein Regulators
Rodentia
Brain
Electrons
Dentate Gyrus
Light
Fluorescent Antibody Technique
Euchromatin
Corpus Striatum
Parvalbumins
Pyramidal Cells
GTP Phosphohydrolases
Microglia
Carisoprodol
Interneurons
Prosencephalon
GTP-Binding Proteins
Synapses
Nervous System

Keywords

  • Hippocampus
  • Localization
  • Microglia
  • Nucleus
  • Parvalbumin
  • Serotonin
  • Striatum

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Regional, cellular, and subcellular localization of RGS10 in rodent brain. / Waugh, Jeff L; Lou, Angela C.; Eisch, Amelia J; Monteggia, Lisa M; Muly, E. Chris; Gold, Stephen J.

In: Journal of Comparative Neurology, Vol. 481, No. 3, 17.01.2005, p. 299-313.

Research output: Contribution to journalArticle

Waugh, Jeff L ; Lou, Angela C. ; Eisch, Amelia J ; Monteggia, Lisa M ; Muly, E. Chris ; Gold, Stephen J. / Regional, cellular, and subcellular localization of RGS10 in rodent brain. In: Journal of Comparative Neurology. 2005 ; Vol. 481, No. 3. pp. 299-313.
@article{0713390c193c476685876c11d39ff747,
title = "Regional, cellular, and subcellular localization of RGS10 in rodent brain",
abstract = "The regulator of G protein signaling type 10 (RGS10) modulates Gαi/o signaling by means of its GTPase accelerating activity and is abundantly expressed in brain and in immune tissues. To elucidate RGS10 function in the nervous system, we mapped RGS10 protein in rat and mouse brain using light microscopic (LM) and electron microscopic (EM) immunohistochemical techniques. The LM showed that RGS10-like immunoreactivity (LIR) labels all cellular subcompartments of neurons and microglia, including their nuclei. There were several differences between RGS10-LIR distributions in rat and mouse, the most striking of which were the far denser immunoreactivity in rat dentate gyrus and dorsal raphe. The EM analysis corroborated and extended our findings from LM. Thus, EM confirmed the presence of dense RGS10-LIR in the euchromatin compartment of nuclei. The EM analysis also resolved dense staining on terminals at symmetric synapses onto pyramidal cell somata. Dual immunofluorescence showed that forebrain interneurons densely labeled with RGS10-LIR partially colocalized with parvalbumin-LIR. Dual-labeling histochemistry in caudoputamen demonstrated that densely labeled striatal cells were biased to the indirect-projecting output pathway. Dual-labeling immunofluorescence also showed that densely labeled RGS10-LIR cells in the dentate gyrus subgranular zone were not proliferating but that newly born cells could differentiate to express RGS10-LIR. Taken together, these data support a role for RGS10 in diverse processes that include modulation of pre- and postsynaptic G-protein signaling. Moreover, enrichment of RGS10 in transcriptionally active regions of the nucleus suggests an unforeseen role of RGS10 in modulating gene expression.",
keywords = "Hippocampus, Localization, Microglia, Nucleus, Parvalbumin, Serotonin, Striatum",
author = "Waugh, {Jeff L} and Lou, {Angela C.} and Eisch, {Amelia J} and Monteggia, {Lisa M} and Muly, {E. Chris} and Gold, {Stephen J.}",
year = "2005",
month = "1",
day = "17",
doi = "10.1002/cne.20372",
language = "English (US)",
volume = "481",
pages = "299--313",
journal = "Journal of Comparative Neurology",
issn = "0021-9967",
publisher = "Wiley-Liss Inc.",
number = "3",

}

TY - JOUR

T1 - Regional, cellular, and subcellular localization of RGS10 in rodent brain

AU - Waugh, Jeff L

AU - Lou, Angela C.

AU - Eisch, Amelia J

AU - Monteggia, Lisa M

AU - Muly, E. Chris

AU - Gold, Stephen J.

PY - 2005/1/17

Y1 - 2005/1/17

N2 - The regulator of G protein signaling type 10 (RGS10) modulates Gαi/o signaling by means of its GTPase accelerating activity and is abundantly expressed in brain and in immune tissues. To elucidate RGS10 function in the nervous system, we mapped RGS10 protein in rat and mouse brain using light microscopic (LM) and electron microscopic (EM) immunohistochemical techniques. The LM showed that RGS10-like immunoreactivity (LIR) labels all cellular subcompartments of neurons and microglia, including their nuclei. There were several differences between RGS10-LIR distributions in rat and mouse, the most striking of which were the far denser immunoreactivity in rat dentate gyrus and dorsal raphe. The EM analysis corroborated and extended our findings from LM. Thus, EM confirmed the presence of dense RGS10-LIR in the euchromatin compartment of nuclei. The EM analysis also resolved dense staining on terminals at symmetric synapses onto pyramidal cell somata. Dual immunofluorescence showed that forebrain interneurons densely labeled with RGS10-LIR partially colocalized with parvalbumin-LIR. Dual-labeling histochemistry in caudoputamen demonstrated that densely labeled striatal cells were biased to the indirect-projecting output pathway. Dual-labeling immunofluorescence also showed that densely labeled RGS10-LIR cells in the dentate gyrus subgranular zone were not proliferating but that newly born cells could differentiate to express RGS10-LIR. Taken together, these data support a role for RGS10 in diverse processes that include modulation of pre- and postsynaptic G-protein signaling. Moreover, enrichment of RGS10 in transcriptionally active regions of the nucleus suggests an unforeseen role of RGS10 in modulating gene expression.

AB - The regulator of G protein signaling type 10 (RGS10) modulates Gαi/o signaling by means of its GTPase accelerating activity and is abundantly expressed in brain and in immune tissues. To elucidate RGS10 function in the nervous system, we mapped RGS10 protein in rat and mouse brain using light microscopic (LM) and electron microscopic (EM) immunohistochemical techniques. The LM showed that RGS10-like immunoreactivity (LIR) labels all cellular subcompartments of neurons and microglia, including their nuclei. There were several differences between RGS10-LIR distributions in rat and mouse, the most striking of which were the far denser immunoreactivity in rat dentate gyrus and dorsal raphe. The EM analysis corroborated and extended our findings from LM. Thus, EM confirmed the presence of dense RGS10-LIR in the euchromatin compartment of nuclei. The EM analysis also resolved dense staining on terminals at symmetric synapses onto pyramidal cell somata. Dual immunofluorescence showed that forebrain interneurons densely labeled with RGS10-LIR partially colocalized with parvalbumin-LIR. Dual-labeling histochemistry in caudoputamen demonstrated that densely labeled striatal cells were biased to the indirect-projecting output pathway. Dual-labeling immunofluorescence also showed that densely labeled RGS10-LIR cells in the dentate gyrus subgranular zone were not proliferating but that newly born cells could differentiate to express RGS10-LIR. Taken together, these data support a role for RGS10 in diverse processes that include modulation of pre- and postsynaptic G-protein signaling. Moreover, enrichment of RGS10 in transcriptionally active regions of the nucleus suggests an unforeseen role of RGS10 in modulating gene expression.

KW - Hippocampus

KW - Localization

KW - Microglia

KW - Nucleus

KW - Parvalbumin

KW - Serotonin

KW - Striatum

UR - http://www.scopus.com/inward/record.url?scp=11144275311&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=11144275311&partnerID=8YFLogxK

U2 - 10.1002/cne.20372

DO - 10.1002/cne.20372

M3 - Article

C2 - 15593368

AN - SCOPUS:11144275311

VL - 481

SP - 299

EP - 313

JO - Journal of Comparative Neurology

JF - Journal of Comparative Neurology

SN - 0021-9967

IS - 3

ER -