Regional tumor oxygen dynamics: 19F PBSR EPI of hexafluorobenzene

Dennis Le, Ralph P. Mason, Sandeep Hunjan, Anca Constantinescu, Bruce R. Barker, Peter P. Antich

Research output: Contribution to journalArticle

49 Citations (Scopus)

Abstract

We demonstrate a novel approach to measuring regional tumor oxygen tension using 19F pulse burst saturation recovery echo planar imaging (EPI) relaxometry of hexaflurobenzene. Hexafluorobenzene offers exceptional sensitivity to changes in oxygen tension, and has a single resonance making it ideal for imaging studies. By combining a pulse burst saturation recovery preparation sequence with EPI, the relaxation experiments were performed in ~20 min facilitating measurements of dynamic changes in pO2 accompanying interventions. Direct intratumoral administration of hexafluorobenzene permitted labeling of specific regions of interest and imaging provided maps of pO2, confirming distinct intra tumoral heterogeneity. For a group of three Dunning prostate adenocarcinoma R3327-AT1 tumors interrogation of the central tumor region showed skewed pO2 distributions with considerable radiobiological hypoxia (~90% voxels had pO2 < 15 torr) when rats breathed 33% O2. Altering the inspired gas to pure oxygen caused distributions to shift towards increased pO2 with significant increases in mean oxygen tension (p < 0.05) in two cases. Interrogation of both central and peripheral regions in a fourth tumor showed bimodal distribution for tumor oxygenation including ~75% voxels with pO2 > 15 tort. EPI allows the fate of individual voxels to be traced: upon altering the inspired gas to pure oxygen those voxels with baseline pO2 > 30 torr showed significant changes (p < 0.05), whereas those with pO2 < 16 torr showed minimal response. The precision of the measurements, together with the ability to simultaneously examine dynamic changes in multiple regions should provide a useful technique for investigating tumor hypoxia with respect to therapy.

Original languageEnglish (US)
Pages (from-to)971-981
Number of pages11
JournalMagnetic Resonance Imaging
Volume15
Issue number8
DOIs
StatePublished - 1997

Fingerprint

Echo-Planar Imaging
Tumors
echoes
tumors
oxygen tension
Oxygen
Imaging techniques
hypoxia
oxygen
Legal Liability
bursts
Neoplasms
recovery
saturation
Prostate
interrogation
Adenocarcinoma
Recovery
pulses
Gases

Keywords

  • F magnetic resonance imaging
  • Echo planar imaging (EPI)
  • Hexafluorobenzene
  • Oximetry
  • Tumor

ASJC Scopus subject areas

  • Biophysics
  • Clinical Biochemistry
  • Structural Biology
  • Radiology Nuclear Medicine and imaging
  • Condensed Matter Physics

Cite this

Regional tumor oxygen dynamics : 19F PBSR EPI of hexafluorobenzene. / Le, Dennis; Mason, Ralph P.; Hunjan, Sandeep; Constantinescu, Anca; Barker, Bruce R.; Antich, Peter P.

In: Magnetic Resonance Imaging, Vol. 15, No. 8, 1997, p. 971-981.

Research output: Contribution to journalArticle

Le, D, Mason, RP, Hunjan, S, Constantinescu, A, Barker, BR & Antich, PP 1997, 'Regional tumor oxygen dynamics: 19F PBSR EPI of hexafluorobenzene', Magnetic Resonance Imaging, vol. 15, no. 8, pp. 971-981. https://doi.org/10.1016/S0730-725X(97)00035-0
Le, Dennis ; Mason, Ralph P. ; Hunjan, Sandeep ; Constantinescu, Anca ; Barker, Bruce R. ; Antich, Peter P. / Regional tumor oxygen dynamics : 19F PBSR EPI of hexafluorobenzene. In: Magnetic Resonance Imaging. 1997 ; Vol. 15, No. 8. pp. 971-981.
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