TY - JOUR
T1 - Regulated changes in material properties underlie centrosome disassembly during mitotic exit
AU - Mittasch, Matthäus
AU - Tran, Vanna M.
AU - Rios, Manolo U.
AU - Fritsch, Anatol W.
AU - Enos, Stephen J.
AU - Gomes, Beatriz Ferreira
AU - Bond, Alec
AU - Kreysing, Moritz
AU - Woodruff, Jeffrey B.
N1 - Funding Information:
J.B. Woodruff is supported by a Cancer Prevention and Research Institute of Texas grant (RR170063) and the Endowed Scholars program at University of Texas Southwestern Medical Center. M. Kreysing is supported by the Max Planck Society and European Research Council (grant 853619), and we acknowledge a Deutsche Forschungsgemeinschaft–financed Dresden International PhD Program fellowship for M. Mittasch. The authors declare no competing financial interests.
Publisher Copyright:
© 2020 Mittasch et al.
PY - 2020
Y1 - 2020
N2 - Centrosomes must resist microtubule-mediated forces for mitotic chromosome segregation. During mitotic exit, however, centrosomes are deformed and fractured by those same forces, which is a key step in centrosome disassembly. How the functional material properties of centrosomes change throughout the cell cycle, and how they are molecularly tuned, remain unknown. Here, we used optically induced flow perturbations to determine the molecular basis of centrosome strength and ductility in C. elegans embryos. We found that both properties declined sharply at anaphase onset, long before natural disassembly. This mechanical transition required PP2A phosphatase and correlated with inactivation of PLK-1 (Polo kinase) and SPD-2 (Cep192). In vitro, PLK-1 and SPD-2 directly protected centrosome scaffolds from force-induced disassembly. Our results suggest that, before anaphase, PLK-1 and SPD-2 respectively confer strength and ductility to the centrosome scaffold so that it can resist microtubule-pulling forces. In anaphase, centrosomes lose PLK-1 and SPD-2 and transition to a weak, brittle state that enables force-mediated centrosome disassembly.
AB - Centrosomes must resist microtubule-mediated forces for mitotic chromosome segregation. During mitotic exit, however, centrosomes are deformed and fractured by those same forces, which is a key step in centrosome disassembly. How the functional material properties of centrosomes change throughout the cell cycle, and how they are molecularly tuned, remain unknown. Here, we used optically induced flow perturbations to determine the molecular basis of centrosome strength and ductility in C. elegans embryos. We found that both properties declined sharply at anaphase onset, long before natural disassembly. This mechanical transition required PP2A phosphatase and correlated with inactivation of PLK-1 (Polo kinase) and SPD-2 (Cep192). In vitro, PLK-1 and SPD-2 directly protected centrosome scaffolds from force-induced disassembly. Our results suggest that, before anaphase, PLK-1 and SPD-2 respectively confer strength and ductility to the centrosome scaffold so that it can resist microtubule-pulling forces. In anaphase, centrosomes lose PLK-1 and SPD-2 and transition to a weak, brittle state that enables force-mediated centrosome disassembly.
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U2 - 10.1083/JCB.201912036
DO - 10.1083/JCB.201912036
M3 - Article
C2 - 32050025
AN - SCOPUS:85079337199
SN - 0021-9525
VL - 219
JO - Journal of Cell Biology
JF - Journal of Cell Biology
IS - 4
M1 - e201912036
ER -