Regulation of antibody isotype secretion by subsets of antigen-specific helper T cells

Tracy L. Stevens, Alexis Bossie, Virginia M. Sanders, Rafael Fernandez-Botran, Robert L. Coffman, Timothy R. Mosmann, Ellen S. Vitetta

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Abstract

The regulation of the subclass of immunoglobulin secreted by B cells has been studied in vitro in polyclonal systems using mitogens, such as lipopolysaccharide (LPS), to bypass the requirement for cognate interaction between antigen-specific T and B cells. In these systems, interleukin-(IL)-4 induces the secretion of IgG1, (ref. 1) and IgE (ref. 2); IL-5 enhances the secretion of IgA3,4, and interferon-γ (IFN-γ) enhances the secretion of IgG2a (ref. 5). Clones of murine TH cells can be divided into two subsets, TH1 and TH2 (ref. 6). Both subsets synthesize IL-3 and granulocyte-monocyte colony-stimulating factor (GM-CSF), but only TH1 clones produce IL-2, IFN-γ, and lymphotoxin (LT) and TH2 clones produce IL-4 and IL-5 (ref. 7). We have examined the role of clones of antigen-specific TH1 and TH2 cells in the regulation of the subclasses of IgG antibody secreted by antigen-specific B cells. Our results show that both types of TH cells induce the secretion of IgM and IgG3, whereas clones of TH1 and TH2 cells specifically induce antigen-specific B cells to secrete IgG2a and IgG1, respectively. We also demonstrate that regulation of commitment to the secretion of a particular IgG isotype occurs in two distinct stages: cognate interaction between T and B cells and interaction between T-cell-derived lymphokines and B cells.

Original languageEnglish (US)
Pages (from-to)255-258
Number of pages4
JournalNature
Volume334
Issue number6179
StatePublished - 1988

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Helper-Inducer T-Lymphocytes
B-Lymphocytes
Antigens
Clone Cells
Immunoglobulin G
Antibodies
Interleukin-4
Interleukin-5
T-Lymphocytes
Interferons
Lymphotoxin-alpha
Lymphokines
Interleukin-3
Granulocyte Colony-Stimulating Factor
Mitogens
Cell Communication
Immunoglobulin E
Interleukin-2
Immunoglobulin M
Lipopolysaccharides

ASJC Scopus subject areas

  • General

Cite this

Stevens, T. L., Bossie, A., Sanders, V. M., Fernandez-Botran, R., Coffman, R. L., Mosmann, T. R., & Vitetta, E. S. (1988). Regulation of antibody isotype secretion by subsets of antigen-specific helper T cells. Nature, 334(6179), 255-258.

Regulation of antibody isotype secretion by subsets of antigen-specific helper T cells. / Stevens, Tracy L.; Bossie, Alexis; Sanders, Virginia M.; Fernandez-Botran, Rafael; Coffman, Robert L.; Mosmann, Timothy R.; Vitetta, Ellen S.

In: Nature, Vol. 334, No. 6179, 1988, p. 255-258.

Research output: Contribution to journalArticle

Stevens, TL, Bossie, A, Sanders, VM, Fernandez-Botran, R, Coffman, RL, Mosmann, TR & Vitetta, ES 1988, 'Regulation of antibody isotype secretion by subsets of antigen-specific helper T cells', Nature, vol. 334, no. 6179, pp. 255-258.
Stevens TL, Bossie A, Sanders VM, Fernandez-Botran R, Coffman RL, Mosmann TR et al. Regulation of antibody isotype secretion by subsets of antigen-specific helper T cells. Nature. 1988;334(6179):255-258.
Stevens, Tracy L. ; Bossie, Alexis ; Sanders, Virginia M. ; Fernandez-Botran, Rafael ; Coffman, Robert L. ; Mosmann, Timothy R. ; Vitetta, Ellen S. / Regulation of antibody isotype secretion by subsets of antigen-specific helper T cells. In: Nature. 1988 ; Vol. 334, No. 6179. pp. 255-258.
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