Abstract
Adipose tissue is the major site of estrogen formation in postmenopausal women. We have previously reported (Simpson E.R., Ackerman G.E., Smith M.E. and Mendelson C.R. (1981) Proc. Natl. Acad. Sci. (U.S.A.) 78, 5690-5694; Mendelson C.R., Cleland W.H., Smith M.E. and Simpson E.R. (1982) Endocrinology 111, 1077-1085) that aromatase activity of human adipose stromal cells in culture is stimulated by glucocorticoids and by dibutyryl cyclic AMP (Bt2-cAMP). In order to establish which physiological factors might stimulate aromatase activity of these cells by activation of adenylate cyclase, we have investigated the roles of adrenocorticotropin (ACTH) and isoproterenol to increase cyclic AMP levels and stimulate the aromatization of androstenedione. In the presence of methylisobutylxanthine (MIX), ACTH stimulated cyclic AMP formation and aromatase activity in a time- and concentration-dependent manner. The concentration of ACTH required for half-maximal stimulation was ~ 10-8 M. Isoproterenol, in the presence of MIX, stimulated cyclic AMP formation in a time- and concentration-dependent fashion, and also stimulated aromatase activity. These effects of isoproterenol appeared to be mediated by binding of the agonist to a population of β-adrenergic receptors. On the basis of these and our previous studies, we suggest that ACTH may play an important role in stimulating estrogen formation by human adipose tissue, both directly, and by stimulating the adrenal cortex to produce both substrate, androstenedione, and inducing agent, namely cortisol.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 61-72 |
| Number of pages | 12 |
| Journal | Molecular and Cellular Endocrinology |
| Volume | 37 |
| Issue number | 1 |
| DOIs | |
| State | Published - 1984 |
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Keywords
- adipose tissue
- estrogen formation
- postmenopausal women
ASJC Scopus subject areas
- Endocrinology
- Endocrinology, Diabetes and Metabolism
Cite this
Regulation of aromatase activity of cultured adipose stromal cells by catecholamines and adrenocorticotropin. / Mendelson, Carole R.; Smith, Margaret E.; Cleland, William H.; Simpson, Evan R.
In: Molecular and Cellular Endocrinology, Vol. 37, No. 1, 1984, p. 61-72.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Regulation of aromatase activity of cultured adipose stromal cells by catecholamines and adrenocorticotropin
AU - Mendelson, Carole R.
AU - Smith, Margaret E.
AU - Cleland, William H.
AU - Simpson, Evan R.
PY - 1984
Y1 - 1984
N2 - Adipose tissue is the major site of estrogen formation in postmenopausal women. We have previously reported (Simpson E.R., Ackerman G.E., Smith M.E. and Mendelson C.R. (1981) Proc. Natl. Acad. Sci. (U.S.A.) 78, 5690-5694; Mendelson C.R., Cleland W.H., Smith M.E. and Simpson E.R. (1982) Endocrinology 111, 1077-1085) that aromatase activity of human adipose stromal cells in culture is stimulated by glucocorticoids and by dibutyryl cyclic AMP (Bt2-cAMP). In order to establish which physiological factors might stimulate aromatase activity of these cells by activation of adenylate cyclase, we have investigated the roles of adrenocorticotropin (ACTH) and isoproterenol to increase cyclic AMP levels and stimulate the aromatization of androstenedione. In the presence of methylisobutylxanthine (MIX), ACTH stimulated cyclic AMP formation and aromatase activity in a time- and concentration-dependent manner. The concentration of ACTH required for half-maximal stimulation was ~ 10-8 M. Isoproterenol, in the presence of MIX, stimulated cyclic AMP formation in a time- and concentration-dependent fashion, and also stimulated aromatase activity. These effects of isoproterenol appeared to be mediated by binding of the agonist to a population of β-adrenergic receptors. On the basis of these and our previous studies, we suggest that ACTH may play an important role in stimulating estrogen formation by human adipose tissue, both directly, and by stimulating the adrenal cortex to produce both substrate, androstenedione, and inducing agent, namely cortisol.
AB - Adipose tissue is the major site of estrogen formation in postmenopausal women. We have previously reported (Simpson E.R., Ackerman G.E., Smith M.E. and Mendelson C.R. (1981) Proc. Natl. Acad. Sci. (U.S.A.) 78, 5690-5694; Mendelson C.R., Cleland W.H., Smith M.E. and Simpson E.R. (1982) Endocrinology 111, 1077-1085) that aromatase activity of human adipose stromal cells in culture is stimulated by glucocorticoids and by dibutyryl cyclic AMP (Bt2-cAMP). In order to establish which physiological factors might stimulate aromatase activity of these cells by activation of adenylate cyclase, we have investigated the roles of adrenocorticotropin (ACTH) and isoproterenol to increase cyclic AMP levels and stimulate the aromatization of androstenedione. In the presence of methylisobutylxanthine (MIX), ACTH stimulated cyclic AMP formation and aromatase activity in a time- and concentration-dependent manner. The concentration of ACTH required for half-maximal stimulation was ~ 10-8 M. Isoproterenol, in the presence of MIX, stimulated cyclic AMP formation in a time- and concentration-dependent fashion, and also stimulated aromatase activity. These effects of isoproterenol appeared to be mediated by binding of the agonist to a population of β-adrenergic receptors. On the basis of these and our previous studies, we suggest that ACTH may play an important role in stimulating estrogen formation by human adipose tissue, both directly, and by stimulating the adrenal cortex to produce both substrate, androstenedione, and inducing agent, namely cortisol.
KW - adipose tissue
KW - estrogen formation
KW - postmenopausal women
UR - http://www.scopus.com/inward/record.url?scp=0021261088&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0021261088&partnerID=8YFLogxK
U2 - 10.1016/0303-7207(84)90128-X
DO - 10.1016/0303-7207(84)90128-X
M3 - Article
VL - 37
SP - 61
EP - 72
JO - Molecular and Cellular Endocrinology
JF - Molecular and Cellular Endocrinology
SN - 0303-7207
IS - 1
ER -