Regulation of aromatase activity of cultured adipose stromal cells by catecholamines and adrenocorticotropin

Carole R. Mendelson, Margaret E. Smith, William H. Cleland, Evan R. Simpson

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

Adipose tissue is the major site of estrogen formation in postmenopausal women. We have previously reported (Simpson E.R., Ackerman G.E., Smith M.E. and Mendelson C.R. (1981) Proc. Natl. Acad. Sci. (U.S.A.) 78, 5690-5694; Mendelson C.R., Cleland W.H., Smith M.E. and Simpson E.R. (1982) Endocrinology 111, 1077-1085) that aromatase activity of human adipose stromal cells in culture is stimulated by glucocorticoids and by dibutyryl cyclic AMP (Bt2-cAMP). In order to establish which physiological factors might stimulate aromatase activity of these cells by activation of adenylate cyclase, we have investigated the roles of adrenocorticotropin (ACTH) and isoproterenol to increase cyclic AMP levels and stimulate the aromatization of androstenedione. In the presence of methylisobutylxanthine (MIX), ACTH stimulated cyclic AMP formation and aromatase activity in a time- and concentration-dependent manner. The concentration of ACTH required for half-maximal stimulation was ~ 10-8 M. Isoproterenol, in the presence of MIX, stimulated cyclic AMP formation in a time- and concentration-dependent fashion, and also stimulated aromatase activity. These effects of isoproterenol appeared to be mediated by binding of the agonist to a population of β-adrenergic receptors. On the basis of these and our previous studies, we suggest that ACTH may play an important role in stimulating estrogen formation by human adipose tissue, both directly, and by stimulating the adrenal cortex to produce both substrate, androstenedione, and inducing agent, namely cortisol.

Original languageEnglish (US)
Pages (from-to)61-72
Number of pages12
JournalMolecular and Cellular Endocrinology
Volume37
Issue number1
DOIs
StatePublished - 1984

Fingerprint

Aromatase
Stromal Cells
Adrenocorticotropic Hormone
Catecholamines
Isoproterenol
Cyclic AMP
Androstenedione
Adipose Tissue
Endocrinology
Estrogens
Tissue
Aromatization
Bucladesine
Adrenal Cortex
Adenylyl Cyclases
Human Activities
Adrenergic Receptors
Glucocorticoids
Hydrocortisone
Cell Culture Techniques

Keywords

  • adipose tissue
  • estrogen formation
  • postmenopausal women

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism

Cite this

Regulation of aromatase activity of cultured adipose stromal cells by catecholamines and adrenocorticotropin. / Mendelson, Carole R.; Smith, Margaret E.; Cleland, William H.; Simpson, Evan R.

In: Molecular and Cellular Endocrinology, Vol. 37, No. 1, 1984, p. 61-72.

Research output: Contribution to journalArticle

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