Regulation of aromatase in estrogen-producing cells

Carole R. Mendelson, Claudia T. Evans, Evan R. Simpson

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Human adipose stromal cells in monolayer culture aromatize androstenedione to estrone. The rate of aromatization is stimulated 20- to 30-fold by glucocorticoids when fetal calf serum is present in the culture medium and by dibutyryl cyclic AMP in the absence of serum. The action of dibutyryl cyclic AMP to stimulate aromatase activity is potentiated markedly by phorbol esters and inhibited by growth factors, such as EOF. In order to investigate the mechanisms underlying this multifactorial regulation, we have prepared polyclonal and monoclonal antibodies specific for aromatase cytochrome P-450. By use of these antibodies it was demonstrated that the action of these various factors to regulate aromatase activity was caused by alterations in the rate of synthesis of aromatase cytochrome P-450, whereas the synthesis of the reductase component of the aromatase enzyme complex was relatively unaffected. The changes in the rate of synthesis of aromatase cytochrome P-450 were, in turn, reflective of changes in the levels of translatable mRNA specific for this protein. In order to analyze the levels of aromatase cytochrome P-450 mRNA directly, we have isolated a cloned cDNA insert complementary to the mRNA encoding aromatase cytochrome P-450, by screening a λgt 11 human placental cDNA library utilizing the polyclonal anti-aromatase P-450 IgG. Use of this cDNA probe in Northern analysis of RNA extracted from human adipose stromal cells revealed that the changes in translatable mRNA resulting from incubation of the cells with the various regulatory factors were due to changes in the absolute levels of mRNA encoding this protein.

Original languageEnglish (US)
Pages (from-to)753-757
Number of pages5
JournalJournal of Steroid Biochemistry
Volume27
Issue number4-6
DOIs
StatePublished - 1987

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Aromatase
Estrogens
Cytochrome P-450 Enzyme System
Messenger RNA
Bucladesine
Stromal Cells
Complementary DNA
Aromatization
Androstenedione
Estrone
Phorbol Esters
Serum
Gene Library
Glucocorticoids
Culture Media
Monolayers
Intercellular Signaling Peptides and Proteins
Screening
Oxidoreductases
Proteins

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology

Cite this

Regulation of aromatase in estrogen-producing cells. / Mendelson, Carole R.; Evans, Claudia T.; Simpson, Evan R.

In: Journal of Steroid Biochemistry, Vol. 27, No. 4-6, 1987, p. 753-757.

Research output: Contribution to journalArticle

Mendelson, Carole R. ; Evans, Claudia T. ; Simpson, Evan R. / Regulation of aromatase in estrogen-producing cells. In: Journal of Steroid Biochemistry. 1987 ; Vol. 27, No. 4-6. pp. 753-757.
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