Regulation of Beclin 1 in autophagy

Qiming Sun, Weiliang Fan, Qing Zhong

Research output: Contribution to journalReview articlepeer-review

74 Scopus citations

Abstract

Class III phosphatidylinositol 3-kinase (PI3KC3) plays a pleiotropic role in autophagy and protein sorting pathways. The human core complex of PI3KC3 consists of three major components including PI3KC3/hVps34, p150 and Beclin 1. How the specificity of PI3KC3 complex is derived towards autophagy is not clear. Utilizing a sequential affinity purification coupled with mass spectrometry approach, we have successfully purified a human Beclin 1 complex and cloned a novel protein we called Barkor (Beclin 1-associated autophagy-related key regulator). The function of Barkor in autophagy has been manifested in several assays, including stress-induced LC3 lipidation, autophagosome formation and Salmonella typhimurium amplification. Mechanistically, Barkor competes with UV radiation resistance associated gene product (UVRAG) for interaction with Beclin 1, and orients Beclin 1 to autophagosomes. Barkor shares considerable sequence homology with Atg14 in yeast, representing an evolutionary conserved autophagy specific regulatory step in early autophagosome formation.

Original languageEnglish (US)
Pages (from-to)713-716
Number of pages4
JournalAutophagy
Volume5
Issue number5
DOIs
StatePublished - Jul 1 2009

Keywords

  • Atg14
  • Autophagosome
  • Autophagy
  • Beclin 1
  • Cancer
  • PI3KC3
  • Phosphatidylinositol 3-kinase
  • UVRAG

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

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