Regulation of branching dynamics by axon-intrinsic asymmetries in Tyrosine Kinase Receptor signaling

Marlen Zschätzsch, Carlos Oliva, Marion Langen, Natalie De Geest, Mehmet Neset Ozel, W. Ryan Williamson, William C. Lemon, Alessia Soldano, Sebastian Munck, P. Robin Hiesinger, Natalia Sanchez-Soriano, Bassem A. Hassan

Research output: Contribution to journalArticle

Abstract

Axonal branching allows a neuron to connect to several targets, increasing neuronal circuit complexity. While axonal branching is well described, the mechanisms that control it remain largely unknown. We find that in the Drosophila CNS branches develop through a process of excessive growth followed by pruning. In vivo high-resolution live imaging of developing brains as well as loss and gain of function experiments show that activation of Epidermal Growth Factor Receptor (EGFR) is necessary for branch dynamics and the final branching pattern. Live imaging also reveals that intrinsic asymmetry in EGFR localization regulates the balance between dynamic and static filopodia. Elimination of signaling asymmetry by either loss or gain of EGFR function results in reduced dynamics leading to excessive branch formation. In summary, we propose that the dynamic process of axon branch development is mediated by differential local distribution of signaling receptors. DOI: http://dx.doi.org/10.7554/eLife.01699.001.

Original languageEnglish (US)
Pages (from-to)e01699
JournaleLife
Volume3
DOIs
StatePublished - Apr 22 2014

Fingerprint

Receptor Protein-Tyrosine Kinases
Epidermal Growth Factor Receptor
Axons
Pseudopodia
Imaging techniques
Neuroimaging
Drosophila
Neurons
Brain
Chemical activation
Growth
Networks (circuits)
Experiments

Keywords

  • axonal branching
  • brain development
  • signaling

ASJC Scopus subject areas

  • Neuroscience(all)
  • Medicine(all)
  • Immunology and Microbiology(all)
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Zschätzsch, M., Oliva, C., Langen, M., De Geest, N., Ozel, M. N., Williamson, W. R., ... Hassan, B. A. (2014). Regulation of branching dynamics by axon-intrinsic asymmetries in Tyrosine Kinase Receptor signaling. eLife, 3, e01699. https://doi.org/10.7554/eLife.01699

Regulation of branching dynamics by axon-intrinsic asymmetries in Tyrosine Kinase Receptor signaling. / Zschätzsch, Marlen; Oliva, Carlos; Langen, Marion; De Geest, Natalie; Ozel, Mehmet Neset; Williamson, W. Ryan; Lemon, William C.; Soldano, Alessia; Munck, Sebastian; Hiesinger, P. Robin; Sanchez-Soriano, Natalia; Hassan, Bassem A.

In: eLife, Vol. 3, 22.04.2014, p. e01699.

Research output: Contribution to journalArticle

Zschätzsch, M, Oliva, C, Langen, M, De Geest, N, Ozel, MN, Williamson, WR, Lemon, WC, Soldano, A, Munck, S, Hiesinger, PR, Sanchez-Soriano, N & Hassan, BA 2014, 'Regulation of branching dynamics by axon-intrinsic asymmetries in Tyrosine Kinase Receptor signaling', eLife, vol. 3, pp. e01699. https://doi.org/10.7554/eLife.01699
Zschätzsch M, Oliva C, Langen M, De Geest N, Ozel MN, Williamson WR et al. Regulation of branching dynamics by axon-intrinsic asymmetries in Tyrosine Kinase Receptor signaling. eLife. 2014 Apr 22;3:e01699. https://doi.org/10.7554/eLife.01699
Zschätzsch, Marlen ; Oliva, Carlos ; Langen, Marion ; De Geest, Natalie ; Ozel, Mehmet Neset ; Williamson, W. Ryan ; Lemon, William C. ; Soldano, Alessia ; Munck, Sebastian ; Hiesinger, P. Robin ; Sanchez-Soriano, Natalia ; Hassan, Bassem A. / Regulation of branching dynamics by axon-intrinsic asymmetries in Tyrosine Kinase Receptor signaling. In: eLife. 2014 ; Vol. 3. pp. e01699.
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