Abstract
Axonal branching allows a neuron to connect to several targets, increasing neuronal circuit complexity. While axonal branching is well described, the mechanisms that control it remain largely unknown. We find that in the Drosophila CNS branches develop through a process of excessive growth followed by pruning. In vivo high-resolution live imaging of developing brains as well as loss and gain of function experiments show that activation of Epidermal Growth Factor Receptor (EGFR) is necessary for branch dynamics and the final branching pattern. Live imaging also reveals that intrinsic asymmetry in EGFR localization regulates the balance between dynamic and static filopodia. Elimination of signaling asymmetry by either loss or gain of EGFR function results in reduced dynamics leading to excessive branch formation. In summary, we propose that the dynamic process of axon branch development is mediated by differential local distribution of signaling receptors. DOI: http://dx.doi.org/10.7554/eLife.01699.001.
| Original language | English (US) |
|---|---|
| Pages (from-to) | e01699 |
| Journal | eLife |
| Volume | 3 |
| DOIs | |
| State | Published - Apr 22 2014 |
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Keywords
- axonal branching
- brain development
- signaling
ASJC Scopus subject areas
- Neuroscience(all)
- Medicine(all)
- Immunology and Microbiology(all)
- Biochemistry, Genetics and Molecular Biology(all)
Cite this
Regulation of branching dynamics by axon-intrinsic asymmetries in Tyrosine Kinase Receptor signaling. / Zschätzsch, Marlen; Oliva, Carlos; Langen, Marion; De Geest, Natalie; Ozel, Mehmet Neset; Williamson, W. Ryan; Lemon, William C.; Soldano, Alessia; Munck, Sebastian; Hiesinger, P. Robin; Sanchez-Soriano, Natalia; Hassan, Bassem A.
In: eLife, Vol. 3, 22.04.2014, p. e01699.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Regulation of branching dynamics by axon-intrinsic asymmetries in Tyrosine Kinase Receptor signaling
AU - Zschätzsch, Marlen
AU - Oliva, Carlos
AU - Langen, Marion
AU - De Geest, Natalie
AU - Ozel, Mehmet Neset
AU - Williamson, W. Ryan
AU - Lemon, William C.
AU - Soldano, Alessia
AU - Munck, Sebastian
AU - Hiesinger, P. Robin
AU - Sanchez-Soriano, Natalia
AU - Hassan, Bassem A.
PY - 2014/4/22
Y1 - 2014/4/22
N2 - Axonal branching allows a neuron to connect to several targets, increasing neuronal circuit complexity. While axonal branching is well described, the mechanisms that control it remain largely unknown. We find that in the Drosophila CNS branches develop through a process of excessive growth followed by pruning. In vivo high-resolution live imaging of developing brains as well as loss and gain of function experiments show that activation of Epidermal Growth Factor Receptor (EGFR) is necessary for branch dynamics and the final branching pattern. Live imaging also reveals that intrinsic asymmetry in EGFR localization regulates the balance between dynamic and static filopodia. Elimination of signaling asymmetry by either loss or gain of EGFR function results in reduced dynamics leading to excessive branch formation. In summary, we propose that the dynamic process of axon branch development is mediated by differential local distribution of signaling receptors. DOI: http://dx.doi.org/10.7554/eLife.01699.001.
AB - Axonal branching allows a neuron to connect to several targets, increasing neuronal circuit complexity. While axonal branching is well described, the mechanisms that control it remain largely unknown. We find that in the Drosophila CNS branches develop through a process of excessive growth followed by pruning. In vivo high-resolution live imaging of developing brains as well as loss and gain of function experiments show that activation of Epidermal Growth Factor Receptor (EGFR) is necessary for branch dynamics and the final branching pattern. Live imaging also reveals that intrinsic asymmetry in EGFR localization regulates the balance between dynamic and static filopodia. Elimination of signaling asymmetry by either loss or gain of EGFR function results in reduced dynamics leading to excessive branch formation. In summary, we propose that the dynamic process of axon branch development is mediated by differential local distribution of signaling receptors. DOI: http://dx.doi.org/10.7554/eLife.01699.001.
KW - axonal branching
KW - brain development
KW - signaling
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U2 - 10.7554/eLife.01699
DO - 10.7554/eLife.01699
M3 - Article
C2 - 24755286
VL - 3
SP - e01699
JO - eLife
JF - eLife
SN - 2050-084X
ER -