Regulation of cGMP-induced relaxation and cGMP-dependent protein kinase in rat myometrium during pregnancy

R. Ann Word, Trudy L. Cornwell

Research output: Contribution to journalArticle

45 Citations (Scopus)

Abstract

Increases in guanosine 3',5'-cyclic monophosphate (cGMP) induced by nitric oxide (NO), nitrovasodilators, and atrial peptides correlate with relaxation of vascular smooth muscle. Relaxation of myometrial smooth muscle by increases in cGMP, however, has required unusually high concentrations of the cyclic nucleotide. We tested the hypothesis that the sensitivity of myometrium to relaxation by cGMP is increased during pregnancy. Aortic smooth muscle was more sensitive to relaxation by cGMP than myometrial tissues, and, contrary to our hypothesis, myometrium from pregnant rats was least sensitive. Although levels of cGMP were elevated after treatment with the NO donor, S-nitroso-N-acetylpenicillamine, relaxation of myometrial tissues obtained from pregnant rats occurred only at extraordinarily high concentrations. The levels of cGMP-dependent protein kinase (PKG) were significantly decreased in myometrium from pregnant rats compared with myometrium from nonpregnant cycling animals or aortic smooth muscle. Administration of estradiol to ovariectomized rats increased myometrial PKG expression, and progesterone antagonized this response. We conclude that 1) myometrial tissues from pregnant rats are not sensitive to relaxation by cGMP and 2) this insensitivity to cGMP is accompanied by progesterone-mediated decreases in the level of PKG expression.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Cell Physiology
Volume274
Issue number3 43-3
StatePublished - Mar 1998

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Cyclic GMP-Dependent Protein Kinases
Myometrium
Rats
Muscle
Pregnancy
Smooth Muscle
Tissue
Progesterone
S-Nitroso-N-Acetylpenicillamine
Nitric Oxide Donors
Cyclic Nucleotides
Cyclic GMP
Vascular Smooth Muscle
Estradiol
Nitric Oxide
Animals

Keywords

  • Atrial natriuretic peptide
  • Guanosine 3',5'-cyclic monophosphate phosphodiesterase
  • Nitric oxide
  • Oxytocin
  • Progesterone

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Cell Biology
  • Physiology
  • Physiology (medical)

Cite this

Regulation of cGMP-induced relaxation and cGMP-dependent protein kinase in rat myometrium during pregnancy. / Word, R. Ann; Cornwell, Trudy L.

In: American Journal of Physiology - Cell Physiology, Vol. 274, No. 3 43-3, 03.1998.

Research output: Contribution to journalArticle

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