TY - JOUR
T1 - Regulation of Clathrin-Mediated Endocytosis
AU - Mettlen, Marcel
AU - Chen, Ping Hung
AU - Srinivasan, Saipraveen
AU - Danuser, Gaudenz
AU - Schmid, Sandra L.
N1 - Publisher Copyright:
© 2018 by Annual Reviews. All rights reserved.
PY - 2018/6/20
Y1 - 2018/6/20
N2 - Clathrin-mediated endocytosis (CME) is the major endocytic pathway in mammalian cells. It is responsible for the uptake of transmembrane receptors and transporters, for remodeling plasma membrane composition in response to environmental changes, and for regulating cell surface signaling. CME occurs via the assembly and maturation of clathrin-coated pits that concentrate cargo as they invaginate and pinch off to form clathrin-coated vesicles. In addition to the major coat proteins, clathrin triskelia and adaptor protein complexes, CME requires a myriad of endocytic accessory proteins and phosphatidylinositol lipids. CME is regulated at multiple steps - initiation, cargo selection, maturation, and fission - and is monitored by an endocytic checkpoint that induces disassembly of defective pits. Regulation occurs via posttranslational modifications, allosteric conformational changes, and isoform and splice-variant differences among components of the CME machinery, including the GTPase dynamin. This review summarizes recent findings on the regulation of CME and the evolution of this complex process.
AB - Clathrin-mediated endocytosis (CME) is the major endocytic pathway in mammalian cells. It is responsible for the uptake of transmembrane receptors and transporters, for remodeling plasma membrane composition in response to environmental changes, and for regulating cell surface signaling. CME occurs via the assembly and maturation of clathrin-coated pits that concentrate cargo as they invaginate and pinch off to form clathrin-coated vesicles. In addition to the major coat proteins, clathrin triskelia and adaptor protein complexes, CME requires a myriad of endocytic accessory proteins and phosphatidylinositol lipids. CME is regulated at multiple steps - initiation, cargo selection, maturation, and fission - and is monitored by an endocytic checkpoint that induces disassembly of defective pits. Regulation occurs via posttranslational modifications, allosteric conformational changes, and isoform and splice-variant differences among components of the CME machinery, including the GTPase dynamin. This review summarizes recent findings on the regulation of CME and the evolution of this complex process.
KW - AP2
KW - adaptor protein-2
KW - dynamin
KW - endocytic accessory proteins
KW - endocytic checkpoint
KW - evolution
KW - signaling
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U2 - 10.1146/annurev-biochem-062917-012644
DO - 10.1146/annurev-biochem-062917-012644
M3 - Review article
C2 - 29661000
AN - SCOPUS:85046409140
SN - 0066-4154
VL - 87
SP - 871
EP - 896
JO - Annual review of biochemistry
JF - Annual review of biochemistry
ER -