Regulation of CREB phosphorylation in the suprachiasmatic nucleus by light and a circadian clock

David D. Ginty, Jon M. Kornhauser, Margaret A. Thompson, Hilmar Bading, Kelly E. Mayo, Joseph S. Takahashi, Michael E. Greenberg

Research output: Contribution to journalArticle

690 Citations (Scopus)

Abstract

Mammalian circadian rhythms are regulated by a pacemaker within the suprachiasmatic nuclei (SCN) of the hypothalamus. The molecular mechanisms controlling the synchronization of the circadian pacemaker are unknown; however, immediate early gene (IEG) expression in the SCN is tightly correlated with entrainment of SCN-regulated rhythms. Antibodies were isolated that recognize the activated, phosphorylated form of the transcription factor cyclic adenosine monophosphate response element binding protein (CREB). Within minutes after exposure of hamsters to light, CREB in the SCN became phosphorylated on the transcriptional regulatory site, Ser133. CREB phosphorylation was dependent on circadian time: CREB became phosphorylated only at times during the circadian cycle when light induced IEG expression and caused phase shifts of circadian rhythms. These results implicate CREB in neuronal signaling in the hypothalamus and suggest that circadian clock gating of light-regulated molecular responses in the SCN occurs upstream of phosphorylation of CREB.

Original languageEnglish (US)
Pages (from-to)238-241
Number of pages4
JournalScience
Volume260
Issue number5105
StatePublished - Apr 9 1993

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Circadian Clocks
Suprachiasmatic Nucleus
Phosphorylation
Light
Immediate-Early Genes
Circadian Rhythm
Hypothalamus
CREB-Binding Protein
Gene Expression
Photoperiod
Response Elements
Cricetinae
Cyclic AMP
Transcription Factors
Antibodies

ASJC Scopus subject areas

  • General

Cite this

Ginty, D. D., Kornhauser, J. M., Thompson, M. A., Bading, H., Mayo, K. E., Takahashi, J. S., & Greenberg, M. E. (1993). Regulation of CREB phosphorylation in the suprachiasmatic nucleus by light and a circadian clock. Science, 260(5105), 238-241.

Regulation of CREB phosphorylation in the suprachiasmatic nucleus by light and a circadian clock. / Ginty, David D.; Kornhauser, Jon M.; Thompson, Margaret A.; Bading, Hilmar; Mayo, Kelly E.; Takahashi, Joseph S.; Greenberg, Michael E.

In: Science, Vol. 260, No. 5105, 09.04.1993, p. 238-241.

Research output: Contribution to journalArticle

Ginty, DD, Kornhauser, JM, Thompson, MA, Bading, H, Mayo, KE, Takahashi, JS & Greenberg, ME 1993, 'Regulation of CREB phosphorylation in the suprachiasmatic nucleus by light and a circadian clock', Science, vol. 260, no. 5105, pp. 238-241.
Ginty DD, Kornhauser JM, Thompson MA, Bading H, Mayo KE, Takahashi JS et al. Regulation of CREB phosphorylation in the suprachiasmatic nucleus by light and a circadian clock. Science. 1993 Apr 9;260(5105):238-241.
Ginty, David D. ; Kornhauser, Jon M. ; Thompson, Margaret A. ; Bading, Hilmar ; Mayo, Kelly E. ; Takahashi, Joseph S. ; Greenberg, Michael E. / Regulation of CREB phosphorylation in the suprachiasmatic nucleus by light and a circadian clock. In: Science. 1993 ; Vol. 260, No. 5105. pp. 238-241.
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