Regulation of dense core release from neuroendocrine cells revealed by imaging single exocytic events

J. K. Angleson, A. J. Cochilla, G. Kilic, I. Nussinovitch, W. J. Betz

Research output: Contribution to journalArticle

138 Citations (Scopus)

Abstract

Using FM1-43 fluorescence, we have optically detected single exocytic and endocytic events in rat pituitary lactotrophs. About fifty discrete fluorescent spots abruptly appear around the entire surface of a cell bathed in FM1-43 and high-potassium saline. The spots, which also immunostain for prolactin, reflect the labeling of dense cores as well as membranes of exocytosed secretory granules. Stained cores are not released, but remain attached to the cell and are eventually endocytosed. However, in cells exposed to dopamine (or an analog, bromocriptine), the cores dissolve and are secreted after several seconds. Solubilization of dense cores is mediated through a reduction in cytoplasmic cyclic AMP. Thus, the composition of secretions from individual secretory granules is regulated.

Original languageEnglish (US)
Pages (from-to)440-446
Number of pages7
JournalNature Neuroscience
Volume2
Issue number5
DOIs
StatePublished - May 1999

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Neuroendocrine Cells
Secretory Vesicles
Lactotrophs
Bromocriptine
Endocytosis
Prolactin
Cyclic AMP
Dopamine
Potassium
Fluorescence
Membranes
FM1 43

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Angleson, J. K., Cochilla, A. J., Kilic, G., Nussinovitch, I., & Betz, W. J. (1999). Regulation of dense core release from neuroendocrine cells revealed by imaging single exocytic events. Nature Neuroscience, 2(5), 440-446. https://doi.org/10.1038/8107

Regulation of dense core release from neuroendocrine cells revealed by imaging single exocytic events. / Angleson, J. K.; Cochilla, A. J.; Kilic, G.; Nussinovitch, I.; Betz, W. J.

In: Nature Neuroscience, Vol. 2, No. 5, 05.1999, p. 440-446.

Research output: Contribution to journalArticle

Angleson, JK, Cochilla, AJ, Kilic, G, Nussinovitch, I & Betz, WJ 1999, 'Regulation of dense core release from neuroendocrine cells revealed by imaging single exocytic events', Nature Neuroscience, vol. 2, no. 5, pp. 440-446. https://doi.org/10.1038/8107
Angleson, J. K. ; Cochilla, A. J. ; Kilic, G. ; Nussinovitch, I. ; Betz, W. J. / Regulation of dense core release from neuroendocrine cells revealed by imaging single exocytic events. In: Nature Neuroscience. 1999 ; Vol. 2, No. 5. pp. 440-446.
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