Regulation of eukaryotic phosphatidylinositol-specific phospholipase C and phospholipase D

William D. Singer, H. Alex Brown, Paul C. Sternweis

Research output: Contribution to journalArticle

338 Citations (Scopus)

Abstract

This review focuses on two phospholipase activities involved in eukaryotic signal transduction. The action of the phosphatidylinositol- specific phospholipase C enzymes produces two well-characterized second messengers, inositol 1,4,5-trisphosphate and diacylglycerol. This discussion emphasizes recent advances in elucidation of the mechanisms of regulation and catalysis of the various isoforms of these enzymes. These are especially related to structural information now available for a phospholipase C δ isozyme. Phospholipase D hydrolyzes phospholipids to produce phosphatidic acid and the respective head group. A perspective of selected past studies is related to emerging molecular characterization of purified and cloned phospholipases D. Evidence for various stimulatory agents (two small G protein families, protein kinase C, and phosphoinositides) suggests complex regulatory mechanisms, and some studies suggest a role for this enzyme activity in intracellular membrane traffic.

Original languageEnglish (US)
Pages (from-to)475-509
Number of pages35
JournalAnnual Review of Biochemistry
Volume66
DOIs
StatePublished - 1997

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Phosphoinositide Phospholipase C
Phospholipase D
Signal transduction
Phosphatidic Acids
Inositol 1,4,5-Trisphosphate
Monomeric GTP-Binding Proteins
Phospholipases
Diglycerides
Enzyme activity
Type C Phospholipases
Second Messenger Systems
Enzymes
Phosphatidylinositols
Protein Kinase C
Catalysis
Isoenzymes
Phospholipids
Intracellular Membranes
Protein Isoforms
Membranes

Keywords

  • Lipid-derived second messengers
  • Phospholipase C
  • Phospholipase D
  • PIP
  • Regulation

ASJC Scopus subject areas

  • Biochemistry

Cite this

Regulation of eukaryotic phosphatidylinositol-specific phospholipase C and phospholipase D. / Singer, William D.; Brown, H. Alex; Sternweis, Paul C.

In: Annual Review of Biochemistry, Vol. 66, 1997, p. 475-509.

Research output: Contribution to journalArticle

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