Regulation of growth and morphological modulation of HeLa65 cells in monolayer culture by dibutyryl cyclic AMP, butyrate and their analogs

N. K. Ghosh, S. I. Deutsch, M. J. Griffin, R. P. Cox

Research output: Contribution to journalArticle

30 Scopus citations

Abstract

N6‐O2′‐Dibutyryl adenosine‐3′,5′ monophosphate (DBcAMP) markedly altered the morphology of HeLa cells by increasing average cell size with an increase in total cell protein and RNA. Such effects were not caused by adenosine 3′,5′ monophosphate (cAMP) or related nucleosides and nucleotides. Butyrate, an enzyme catalyzed hydrolysis product of DBcAMP, induced a jagged spindle shape in HeLa cells within 8 hours and then caused them to enlarge and resemble those grown with DBcAMP. These effects were specific for butyrate (C4) and pentanoate (C5) and were not observed with isomers, substituted analogs, or other fatty acid derivatives. These morphological effects were prevented by blocking protein synthesis or by altering the cytoskeleton with Colcemid or cytochalasin B.

Original languageEnglish (US)
Pages (from-to)663-672
Number of pages10
JournalJournal of cellular physiology
Volume86
Issue number2 S
DOIs
StatePublished - Dec 1975

ASJC Scopus subject areas

  • Physiology
  • Clinical Biochemistry
  • Cell Biology

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