@article{ed864895f86c4ed5979a882c91e57525,
title = "Regulation of heterochromatic DNA replication by histone H3 lysine 27 methyltransferases",
abstract = "Multiple pathways prevent DNA replication from occurring more than once per cell cycle1. These pathways block re-replication by strictly controlling the activity of pre-replication complexes, which assemble at specific sites in the genome called origins. Here we show that mutations in the homologous histone 3 lysine 27 (H3K27) monomethyltransferases, ARABIDOPSIS TRITHORAX-RELATED PROTEIN5 (ATXR5) and ATXR6, lead to re-replication of specific genomic locations. Most of these locations correspond to transposons and other repetitive and silent elements of the Arabidopsis genome. These sites also correspond to high levels of H3K27 monomethylation, and mutation of the catalytic SET domain is sufficient to cause the re-replication defect. Mutation of ATXR5 and ATXR6 also causes upregulation of transposon expression and has pleiotropic effects on plant development. These results uncover a novel pathway that prevents over-replication of heterochromatin in Arabidopsis.",
author = "Yannick Jacob and Hume Stroud and Chantal Leblanc and Suhua Feng and Luting Zhuo and Elena Caro and Christiane Hassel and Crisanto Gutierrez and Michaels, {Scott D.} and Jacobsen, {Steven E.}",
note = "Funding Information: Acknowledgements We thank G. Lambert and D. Galbraith for assistance with flow cytometry; Y. Bernatavichute for assistance with ChIP experiments; and M. Pellegrini and S. Cokus for advice on data analyses. Y.J. was supported by a fellowship from Le Fonds Qu{\'e}b{\'e}cois de la Recherche sur la Nature et les Technologies (FQRNT). S.F. is a Howard Hughes Medical Institute Fellow of the Life Science Research Foundation. Research in the Michaels{\textquoteright} laboratory was supported by grants from the National Institutes of Health (GM075060), the Indiana METACyt Initiative of Indiana University, and the Lilly Endowment, Inc. C.G. was supported by grants from the Spanish Ministry of Science and Innovation (BFU2009-9783 and CSD2007-57B). S.E.J. is an investigator of the Howard Hughes Medical Institute.",
year = "2010",
month = aug,
day = "19",
doi = "10.1038/nature09290",
language = "English (US)",
volume = "466",
pages = "987--991",
journal = "Nature",
issn = "0028-0836",
publisher = "Nature Publishing Group",
number = "7309",
}