Regulation of human B cell function by recombinant CD40 ligand and other TNF-related ligands

M. D. Jumper, Y. Nishioka, L. S. Davis, P. E. Lipsky, K. Meek

Research output: Contribution to journalArticle

58 Scopus citations

Abstract

To assess the potential of CD40 ligand (CD40L) and the related molecules CD27 ligand (CD27L), CD30 ligand (CD30L), and membrane TNF-α to stimulate B cell responses, expression of these proteins in the baculovirus system was performed. Sf9 cells expressing these membrane molecules were cultured with normal human B cells and a variety of B cell lines to assess the functional outcome. The signal provided by CD40L promotes aggregation of B cells, stimulates vigorous proliferation, and induces germ-line transcription of downstream heavy chain constant region genes in the absence of cytokine costimulation. In contrast, CD27L, CD30L, and TNF-α had no effects on B cell proliferation. CD27L and TNF-α had no effect on the induction of germ-line transcripts, whereas CD30L consistently inhibited constitutive and CD40L- induced germ-line transcription of the ε gene by B cell lines that express CD30. These results demonstrate that various members of the TNF family exert specific effects on human B cell function, with CD40L and CD30L providing powerful, but opposing, effects on 1ε transcription.

Original languageEnglish (US)
Pages (from-to)2369-2378
Number of pages10
JournalJournal of Immunology
Volume155
Issue number5
StatePublished - 1995

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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    Jumper, M. D., Nishioka, Y., Davis, L. S., Lipsky, P. E., & Meek, K. (1995). Regulation of human B cell function by recombinant CD40 ligand and other TNF-related ligands. Journal of Immunology, 155(5), 2369-2378.