Regulation of interleukin-6 production in human fetal Kupffer cells

W. H. Kutteh, W. E. Rainey, B. R. Carr

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Inflammatory mediators such as interleukin-1 beta (IL-1β), tumour necrosis factor-alpha (TNFα), and interleukin-6 (IL-6) exhibit local autocrine and paracrine effects as well as distant systemic effects on target cells. Human Kupffer cells, the fixed tissue macrophages of the liver, may modulate immune and endocrine function in early fetal development. We purified and cultured human fetal Kupffer cells to investigate the production of the cytokine, IL-6. Fetal Kupffer cells treated with bacterial lipopolysaccharide (LPS) produced IL-6 in a dose-dependent fashion with maximal secretion (1000 pg per 106 cells) observed within 12 h using 10 μg of LPS/ml. Cortisol and dexamethasone, but not oestrogen, progesterone, or testosterone, dramatically suppressed the LPS-stimulated secretion of IL-6 by fetal Kupffer cells. None of the steroids tested altered basal production or enhanced the LPS-stimulated production of IL-6 by fetal Kupffer cells. The inhibition of glucococorticoids could be reversed by the addition of RU 486, indicating that this effect was mediated by the glucocorticoid receptor. These results demonstrate that the production of IL-6 by fetal hepatic macrophages can be activated by LPS and suppressed by glucocorticoids. These studies suggest that Kupffer cells express mature macrophage function in early gestation and would be capable of regulatory roles in the growth and development of the fetus.

Original languageEnglish (US)
Pages (from-to)607-613
Number of pages7
JournalScandinavian Journal of Immunology
Volume33
Issue number5
StatePublished - 1991

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Kupffer Cells
Interleukin-6
Lipopolysaccharides
Macrophages
Mifepristone
Liver
Glucocorticoid Receptors
Fetal Development
Interleukin-1beta
Growth and Development
Dexamethasone
Glucocorticoids
Progesterone
Hydrocortisone
Testosterone
Estrogens
Fetus
Tumor Necrosis Factor-alpha
Steroids
Cytokines

ASJC Scopus subject areas

  • Immunology

Cite this

Regulation of interleukin-6 production in human fetal Kupffer cells. / Kutteh, W. H.; Rainey, W. E.; Carr, B. R.

In: Scandinavian Journal of Immunology, Vol. 33, No. 5, 1991, p. 607-613.

Research output: Contribution to journalArticle

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