Regulation of lung surfactant secretion by microRNA-150

Tingting Weng, Amarjit Mishra, Yujie Guo, Yang Wang, Lijing Su, Chaoqun Huang, Chunling Zhao, Xiao Xiao, Lin Liu

Research output: Contribution to journalArticle

19 Scopus citations

Abstract

P2X7 receptor (P2X7R) is a purinergic ion-channel receptor. We have previously shown that the activation of P2X7R in alveolar type I cells stimulates surfactant secretion in alveolar type II cells. In this study, we determined whether miR-150 regulates P2X7R-mediated surfactant secretion. The miR-150 expression level in alveolar type II cells was much higher than alveolar type I cells, which was inversely correlated with the P2X7R protein level. An adenovirus expressing miR-150 significantly reduced the P2X7R protein expression in E10 cells, an alveolar type I cell line. Furthermore, pre-treatment of E10 cells with the adenovirus reduced the surfactant secretion induced by E10 cell conditioned medium. Our study demonstrates that miR-150 regulates surfactant secretion through P2X7R.

Original languageEnglish (US)
Pages (from-to)586-589
Number of pages4
JournalBiochemical and Biophysical Research Communications
Volume422
Issue number4
DOIs
StatePublished - Jun 15 2012
Externally publishedYes

Keywords

  • MiR-150
  • P2X7R
  • Surfactant secretion

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Fingerprint Dive into the research topics of 'Regulation of lung surfactant secretion by microRNA-150'. Together they form a unique fingerprint.

  • Cite this

    Weng, T., Mishra, A., Guo, Y., Wang, Y., Su, L., Huang, C., Zhao, C., Xiao, X., & Liu, L. (2012). Regulation of lung surfactant secretion by microRNA-150. Biochemical and Biophysical Research Communications, 422(4), 586-589. https://doi.org/10.1016/j.bbrc.2012.05.030