Abstract
P2X7 receptor (P2X7R) is a purinergic ion-channel receptor. We have previously shown that the activation of P2X7R in alveolar type I cells stimulates surfactant secretion in alveolar type II cells. In this study, we determined whether miR-150 regulates P2X7R-mediated surfactant secretion. The miR-150 expression level in alveolar type II cells was much higher than alveolar type I cells, which was inversely correlated with the P2X7R protein level. An adenovirus expressing miR-150 significantly reduced the P2X7R protein expression in E10 cells, an alveolar type I cell line. Furthermore, pre-treatment of E10 cells with the adenovirus reduced the surfactant secretion induced by E10 cell conditioned medium. Our study demonstrates that miR-150 regulates surfactant secretion through P2X7R.
Original language | English (US) |
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Pages (from-to) | 586-589 |
Number of pages | 4 |
Journal | Biochemical and Biophysical Research Communications |
Volume | 422 |
Issue number | 4 |
DOIs | |
State | Published - Jun 15 2012 |
Externally published | Yes |
Keywords
- MiR-150
- P2X7R
- Surfactant secretion
ASJC Scopus subject areas
- Biophysics
- Biochemistry
- Molecular Biology
- Cell Biology