Regulation of maternal Wnt mRNA translation in C. elegans embryos

Marieke Oldenbroek, Scott M. Robertson, Tugba Guven-Ozkan, Caroline Spike, David Greenstein, Rueyling Lin

Research output: Contribution to journalArticle

16 Scopus citations

Abstract

The restricted spatiotemporal translation of maternal mRNAs, which is crucial for correct cell fate specification in early C. elegans embryos, is regulated primarily through the 3′UTR. Although genetic screens have identified many maternally expressed cell fate-controlling RNA-binding proteins (RBPs), their in vivo targets and the mechanism(s) by which they regulate these targets are less clear. These RBPs are translated in oocytes and localize to one or a few blastomeres in a spatially and temporally dynamic fashion unique for each protein and each blastomere. Here, we characterize the translational regulation of maternally supplied mom-2 mRNA, which encodes a Wnt ligand essential for two separate cell-cell interactions in early embryos. A GFP reporter that includes only the mom-2 3′UTR is translationally repressed properly in oocytes and early embryos, and then correctly translated only in the known Wnt signaling cells. We show that the spatiotemporal translation pattern of this reporter is regulated combinatorially by a set of nine maternally supplied RBPs. These nine proteins all directly bind the mom-2 3′UTR in vitro and function as positive or negative regulators of mom-2 translation in vivo. The net translational readout for the mom-2 3′UTR reporter is determined by competitive binding between positive-and negative-acting RBPs for the 3′UTR, along with the distinct spatiotemporal localization patterns of these regulators. We propose that the 3′UTR of maternal mRNAs contains a combinatorial code that determines the topography of associated RBPs, integrating positive and negative translational inputs.

Original languageEnglish (US)
Pages (from-to)4614-4623
Number of pages10
JournalDevelopment (Cambridge)
Volume140
Issue number22
DOIs
StatePublished - Nov 15 2013

Keywords

  • 3′UTR
  • Caenorhabditis elegans
  • Wnt

ASJC Scopus subject areas

  • Molecular Biology
  • Developmental Biology

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    Oldenbroek, M., Robertson, S. M., Guven-Ozkan, T., Spike, C., Greenstein, D., & Lin, R. (2013). Regulation of maternal Wnt mRNA translation in C. elegans embryos. Development (Cambridge), 140(22), 4614-4623. https://doi.org/10.1242/dev.096313