Regulation of microtubule dynamics and myogenic differentiation by MURF, a striated muscle RING-finger protein

Jeffrey A. Spencer, Susan Eliazer, Robert L. Ilaria, James A. Richardson, Eric N. Olson

Research output: Contribution to journalArticle

136 Citations (Scopus)

Abstract

The RING-finger domain is a novel zinc-binding Cys-His protein motif found in a growing number of proteins involved in signal transduction, ubiquitination, gene transcription, differentiation, and morphogenesis. We describe a novel muscle-specific RING-finger protein (MURF) expressed specifically in cardiac and skeletal muscle cells throughout pre- and postnatal mouse development. MURF belongs to the RING-B-box-coiled-coil subclass of RING-finger proteins, characterized by an NH2-terminal RING-finger followed by a zinc-finger domain (B-box) and a leucine-rich coiled-coil domain. Expression of MURF is required for skeletal myoblast differentiation and myotube fusion. The leucine-rich coiled-coil domain of MURF mediates association with microtubules, whereas the RING-finger domain is required for microtubule stabilization and an additional region is required for homo-oligomerization. Expression of MURF establishes a cellular microtubule network that is resistant to microtubule depolymerization induced by alkaloids, cold and calcium. These results identify MURF as a myogenic regulator of the microtubule network of striated muscle cells and reveal a link between microtubule organization and myogenesis.

Original languageEnglish (US)
Pages (from-to)771-784
Number of pages14
JournalJournal of Cell Biology
Volume150
Issue number4
DOIs
StatePublished - Aug 21 2000

Fingerprint

Striated Muscle
Microtubules
Fingers
Muscles
Proteins
RING Finger Domains
Leucine
Skeletal Myoblasts
Amino Acid Motifs
Muscle Development
Ubiquitination
Zinc Fingers
Skeletal Muscle Fibers
Morphogenesis
Alkaloids
Cardiac Myocytes
Muscle Cells
Zinc
Signal Transduction
Skeletal Muscle

Keywords

  • Cytoskeleton
  • Microtubule
  • RING-finger
  • Striated muscle

ASJC Scopus subject areas

  • Cell Biology

Cite this

Regulation of microtubule dynamics and myogenic differentiation by MURF, a striated muscle RING-finger protein. / Spencer, Jeffrey A.; Eliazer, Susan; Ilaria, Robert L.; Richardson, James A.; Olson, Eric N.

In: Journal of Cell Biology, Vol. 150, No. 4, 21.08.2000, p. 771-784.

Research output: Contribution to journalArticle

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