Regulation of motility in bovine brain endothelial cells

Eliot M. Rosen, Susan Jaken, William Carley, Peter M. Luckett, Eva Setter, Madhu Bhargava, Itzhak D. Goldberg

Research output: Contribution to journalArticle

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Abstract

Scatter factor (SF) is a fibroblast‐derived cytokine which stimulates motility of epithelial and vascular endothelial cells. We used a quantitative assay based on migration of cells from microcarrier beads to flat surfaces to study the regulation of motility in bovine brain endothelial cells (BBEC). Peptide growth factors (EGF, ECGF, basic FGF) did not stimulate migration. Tumor promoting phorbol esters (PMA, PDD) markedly stimulated migration, while inactive phorbol esters (4a‐PDD, phorbol‐13,20‐diacetate) did not affect migration. Both SF‐ and PMA‐stimulated migration were inhibited by (1) TGF‐beta; (2) protein kinase inhibitors (e.g., staurosporine, K‐252a); (3) activators of the adenylate cyclase signaling pathway (e.g., dibutyryl cyclic AMP, theophylline); (4) cycloheximide; and (5) anti‐cytoskeleton agents (e.g., cytochalasin B, colcemid). However, PMA and SF pathways were distinguishable: (1) PMA induced additional migration at saturating SF concentrations; (2) the onset of migration‐stimulation was immediate for PMA and delayed for SF; and (3) down‐modulation of protein kinase C (PKC) ablated PMA but not SF responsiveness. Assessment of PKC by (3H)‐phorbol ester (PDBu) binding and by immunoblot showed (1) scatter factor does not cause significant redistribution or down‐modulation of PDBu binding or alpha‐PKC; and (2) PDBu mediates redistribution and down‐modulation of both binding and alpha‐PKC. These findings suggest two pathways for BBEC motility: a PKC‐dependent pathway and an SF‐stimulated/PKC‐independent pathway.

Original languageEnglish (US)
Pages (from-to)325-335
Number of pages11
JournalJournal of Cellular Physiology
Volume146
Issue number2
DOIs
StatePublished - 1991

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Hepatocyte Growth Factor
Endothelial cells
Brain
Endothelial Cells
Phorbol Esters
Protein Kinase C
Cell Movement
Demecolcine
Bucladesine
Cytochalasin B
Staurosporine
Cycloheximide
Protein Kinase Inhibitors
Theophylline
Adenylyl Cyclases
Transforming Growth Factor beta
Tumors
Assays
Intercellular Signaling Peptides and Proteins
Cells

ASJC Scopus subject areas

  • Medicine(all)
  • Physiology
  • Clinical Biochemistry
  • Cell Biology

Cite this

Rosen, E. M., Jaken, S., Carley, W., Luckett, P. M., Setter, E., Bhargava, M., & Goldberg, I. D. (1991). Regulation of motility in bovine brain endothelial cells. Journal of Cellular Physiology, 146(2), 325-335. https://doi.org/10.1002/jcp.1041460218

Regulation of motility in bovine brain endothelial cells. / Rosen, Eliot M.; Jaken, Susan; Carley, William; Luckett, Peter M.; Setter, Eva; Bhargava, Madhu; Goldberg, Itzhak D.

In: Journal of Cellular Physiology, Vol. 146, No. 2, 1991, p. 325-335.

Research output: Contribution to journalArticle

Rosen, EM, Jaken, S, Carley, W, Luckett, PM, Setter, E, Bhargava, M & Goldberg, ID 1991, 'Regulation of motility in bovine brain endothelial cells', Journal of Cellular Physiology, vol. 146, no. 2, pp. 325-335. https://doi.org/10.1002/jcp.1041460218
Rosen, Eliot M. ; Jaken, Susan ; Carley, William ; Luckett, Peter M. ; Setter, Eva ; Bhargava, Madhu ; Goldberg, Itzhak D. / Regulation of motility in bovine brain endothelial cells. In: Journal of Cellular Physiology. 1991 ; Vol. 146, No. 2. pp. 325-335.
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