Regulation of mouse submaxillary gland renin by thyroxine

C. M. Wilson, M. J. Myhre, R. C. Reynolds, J. D. Wilson

Research output: Contribution to journalArticle

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Abstract

The difference in submaxillary gland (SMG) renin activity of male and female mice is mediated by androgen. However, because hypophysectomy causes a profound decrease in basal renin activity and a diminished response to treatment with androgen, the endocrine regulation of SMG renin must involve a hormone(s) in addition to androgen. To explore the relationship between SMG renin activity and pituitary hormones, we injected hypophysectomized or intact female mice with dihydrotestosterone (DHT), T4, and bovine GH singly and in combination. T4 and DHT administered together produced renin specific activity levels in hypophysectomized mice identical to those in intact mice treated with DHT or DHT plus T4. GH had little or no effect on SMG renin activity in intact or hypophysectomized mice either alone or in combination with DHT and/or T4. T4 acted independently of androgen, inducing SMG renin in Tfm/Y mice that are resistant to DHT because they lack functional androgen receptors. T4 also acted independently of the renin regulator (Rnr) gene, inducing SMG renin in mice that carry b or s alleles at the Rnr locus. Maximum SMG renin activity is determined by Rnr, however, and remained, in C57BL/6J mice (Rnrb/Rnrb) given T4 and/or DHT, 1/100th that in identically treated SWR/J mice (Rnr(s)/Rnr(s)). Mouse SMG renin is localized in secretory granules of the granular convoluted tubule cells. Granular convoluted tubule cells do not mature in untreated mice hypophysectomized at 3 weeks of age, and treatment with both T4 and DHT is required to restore the SMG duct system to acini ratio and the granule content of hypophysectomized mice to the levels found in identically treated intact mice. These results establish a major role for T4 in the regulaton of SMG renin activity in the mouse and demonstrate that the young hypophysectomized mouse is a useful model in which to examine hormonal interaction in the regulation of gene expression.

Original languageEnglish (US)
Pages (from-to)982-989
Number of pages8
JournalEndocrinology
Volume110
Issue number3
StatePublished - 1982

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Submandibular Gland
Thyroxine
Renin
Dihydrotestosterone
Androgens
Hypophysectomy
Pituitary Hormones
Secretory Vesicles
Androgen Receptors
Gene Expression Regulation
Regulator Genes
Inbred C57BL Mouse

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism

Cite this

Wilson, C. M., Myhre, M. J., Reynolds, R. C., & Wilson, J. D. (1982). Regulation of mouse submaxillary gland renin by thyroxine. Endocrinology, 110(3), 982-989.

Regulation of mouse submaxillary gland renin by thyroxine. / Wilson, C. M.; Myhre, M. J.; Reynolds, R. C.; Wilson, J. D.

In: Endocrinology, Vol. 110, No. 3, 1982, p. 982-989.

Research output: Contribution to journalArticle

Wilson, CM, Myhre, MJ, Reynolds, RC & Wilson, JD 1982, 'Regulation of mouse submaxillary gland renin by thyroxine', Endocrinology, vol. 110, no. 3, pp. 982-989.
Wilson CM, Myhre MJ, Reynolds RC, Wilson JD. Regulation of mouse submaxillary gland renin by thyroxine. Endocrinology. 1982;110(3):982-989.
Wilson, C. M. ; Myhre, M. J. ; Reynolds, R. C. ; Wilson, J. D. / Regulation of mouse submaxillary gland renin by thyroxine. In: Endocrinology. 1982 ; Vol. 110, No. 3. pp. 982-989.
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