Regulation of murine class I genes by interferons is controlled by regions located both 5' and 3' to the transcription initiation site

B. Korber, L. Hood, I. Stroynowski

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Abstract

Interferons regulate the expression of a large number of mammalian genes, including the major histocompatibility antigen genes. To investigate the mechanisms involved in interferon action, we have analyzed the ability of murine H-2L(d) and H-2D(d) DNA sequences to control the responses to interferon. The results indicate that interferon regulation of class I gene expression is complex and involves at least two mechanisms that are dependent on class I sequences located upstream and downstream to the transcription initiation site. In transfected mouse L cells, both of these regions are required for full enhancement of class I gene expression, with the major portion of the response controlled by the sequences located 3' to the transcription initiation site. The fine-mapping analysis of the 5' region-encoded response also suggests that recombinant α and γ interferons may exert their effects on class I gene expression by using different cis-acting regulatory sequences.

Original languageEnglish (US)
Pages (from-to)3380-3384
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume84
Issue number10
Publication statusPublished - 1987

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ASJC Scopus subject areas

  • General
  • Genetics

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