Regulation of murine class I genes by interferons is controlled by regions located both 5' and 3' to the transcription initiation site

B. Korber, L. Hood, I. Stroynowski

Research output: Contribution to journalArticle

59 Citations (Scopus)

Abstract

Interferons regulate the expression of a large number of mammalian genes, including the major histocompatibility antigen genes. To investigate the mechanisms involved in interferon action, we have analyzed the ability of murine H-2L(d) and H-2D(d) DNA sequences to control the responses to interferon. The results indicate that interferon regulation of class I gene expression is complex and involves at least two mechanisms that are dependent on class I sequences located upstream and downstream to the transcription initiation site. In transfected mouse L cells, both of these regions are required for full enhancement of class I gene expression, with the major portion of the response controlled by the sequences located 3' to the transcription initiation site. The fine-mapping analysis of the 5' region-encoded response also suggests that recombinant α and γ interferons may exert their effects on class I gene expression by using different cis-acting regulatory sequences.

Original languageEnglish (US)
Pages (from-to)3380-3384
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume84
Issue number10
StatePublished - 1987

Fingerprint

MHC Class I Genes
Transcription Initiation Site
Interferons
Gene Expression
Histocompatibility Antigens
Genes

ASJC Scopus subject areas

  • General
  • Genetics

Cite this

@article{7dacc98f9f9442d993fd9276d7ff90d3,
title = "Regulation of murine class I genes by interferons is controlled by regions located both 5' and 3' to the transcription initiation site",
abstract = "Interferons regulate the expression of a large number of mammalian genes, including the major histocompatibility antigen genes. To investigate the mechanisms involved in interferon action, we have analyzed the ability of murine H-2L(d) and H-2D(d) DNA sequences to control the responses to interferon. The results indicate that interferon regulation of class I gene expression is complex and involves at least two mechanisms that are dependent on class I sequences located upstream and downstream to the transcription initiation site. In transfected mouse L cells, both of these regions are required for full enhancement of class I gene expression, with the major portion of the response controlled by the sequences located 3' to the transcription initiation site. The fine-mapping analysis of the 5' region-encoded response also suggests that recombinant α and γ interferons may exert their effects on class I gene expression by using different cis-acting regulatory sequences.",
author = "B. Korber and L. Hood and I. Stroynowski",
year = "1987",
language = "English (US)",
volume = "84",
pages = "3380--3384",
journal = "Proceedings of the National Academy of Sciences of the United States of America",
issn = "0027-8424",
number = "10",

}

TY - JOUR

T1 - Regulation of murine class I genes by interferons is controlled by regions located both 5' and 3' to the transcription initiation site

AU - Korber, B.

AU - Hood, L.

AU - Stroynowski, I.

PY - 1987

Y1 - 1987

N2 - Interferons regulate the expression of a large number of mammalian genes, including the major histocompatibility antigen genes. To investigate the mechanisms involved in interferon action, we have analyzed the ability of murine H-2L(d) and H-2D(d) DNA sequences to control the responses to interferon. The results indicate that interferon regulation of class I gene expression is complex and involves at least two mechanisms that are dependent on class I sequences located upstream and downstream to the transcription initiation site. In transfected mouse L cells, both of these regions are required for full enhancement of class I gene expression, with the major portion of the response controlled by the sequences located 3' to the transcription initiation site. The fine-mapping analysis of the 5' region-encoded response also suggests that recombinant α and γ interferons may exert their effects on class I gene expression by using different cis-acting regulatory sequences.

AB - Interferons regulate the expression of a large number of mammalian genes, including the major histocompatibility antigen genes. To investigate the mechanisms involved in interferon action, we have analyzed the ability of murine H-2L(d) and H-2D(d) DNA sequences to control the responses to interferon. The results indicate that interferon regulation of class I gene expression is complex and involves at least two mechanisms that are dependent on class I sequences located upstream and downstream to the transcription initiation site. In transfected mouse L cells, both of these regions are required for full enhancement of class I gene expression, with the major portion of the response controlled by the sequences located 3' to the transcription initiation site. The fine-mapping analysis of the 5' region-encoded response also suggests that recombinant α and γ interferons may exert their effects on class I gene expression by using different cis-acting regulatory sequences.

UR - http://www.scopus.com/inward/record.url?scp=0023238926&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0023238926&partnerID=8YFLogxK

M3 - Article

C2 - 3106967

AN - SCOPUS:0023238926

VL - 84

SP - 3380

EP - 3384

JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

SN - 0027-8424

IS - 10

ER -