Regulation of purinergic signaling in biliary epithelial cells by exocytosis of SLC17A9-dependent ATP-enriched vesicles

Meghana N. Sathe, Kangmee Woo, Charles Kresge, Abhijit Bugde, Kate Luby-Phelps, Matthew A. Lewis, Andrew P. Feranchak

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

ATP in bile is a potent secretogogue, stimulating biliary epithelial cell (BEC) secretion through binding apical purinergic receptors. In response to mechanosensitive stimuli, BECs release ATP into bile, although the cellular basis of ATP release is unknown. The aims of this study in human and mouse BECs were to determine whether ATP release occurs via exocytosis of ATP-enriched vesicles and to elucidate the potential role of the vesicular nucleotide transporter SLC17A9 in purinergic signaling. Dynamic, multiscale, live cell imaging (confocal and total internal reflection fluorescence microscopy and a luminescence detection system with a high sensitivity charge-coupled device camera) was utilized to detect vesicular ATP release from cell populations, single cells, and the submembrane space of a single cell. In response to increases in cell volume, BECs release ATP, which was dependent on intact microtubules and vesicular trafficking pathways. ATP release occurred as stochastic point source bursts of luminescence consistent with exocytic events. Parallel studies identified ATP-enriched vesicles ranging in size from 0.4 to 1 μm that underwent fusion and release in response to increases in cell volume in a protein kinase C-dependent manner. Present in all models, SLC17A9 contributed to ATP vesicle formation and regulated ATP release. The findings are consistent with the existence of an SLC17A9-dependent ATP-enriched vesicular pool in biliary epithelium that undergoes regulated exocytosis to initiate purinergic signaling.

Original languageEnglish (US)
Pages (from-to)25363-25376
Number of pages14
JournalJournal of Biological Chemistry
Volume286
Issue number28
DOIs
StatePublished - Jul 15 2011

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Exocytosis
Adenosine Triphosphate
Epithelial Cells
Luminescence
Cell Size
Bile
Purinergic Receptors
Fluorescence microscopy
CCD cameras
Fluorescence Microscopy
Microtubules
Protein Kinase C
Fusion reactions
Epithelium
Nucleotides
Cells

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Molecular Biology

Cite this

Regulation of purinergic signaling in biliary epithelial cells by exocytosis of SLC17A9-dependent ATP-enriched vesicles. / Sathe, Meghana N.; Woo, Kangmee; Kresge, Charles; Bugde, Abhijit; Luby-Phelps, Kate; Lewis, Matthew A.; Feranchak, Andrew P.

In: Journal of Biological Chemistry, Vol. 286, No. 28, 15.07.2011, p. 25363-25376.

Research output: Contribution to journalArticle

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