Regulation of RAD53 by the ATM-like kinases MEC1 and TEL1 in yeast cell cycle checkpoint pathways

Yolanda Sanchez, Brian A. Desany, William J. Jones, Qinghua Liu, Bin Wang, Stephen J. Elledge

Research output: Contribution to journalArticle

495 Citations (Scopus)

Abstract

Mutants of the Saccharomyces cerevisiae ataxia telangiectasia mutated (ATM) homolog MEC1/SAD3/ESR1 were identified that could live only if the RAD53/SAD1 checkpoint kinase was overproduced. MEC1 and a structurally related gene, TEL1, have overlapping functions in response to DNA damage and replication blocks that in mutants can be provided by overproduction of RAD53. Both MEC1 and TEL1 were found to control phosphorylation of Rad53p in response to DNA damage. These results indicate that RAD53 is a signal transducer in the DNA damage and replication checkpoint pathways and functions downstream of two members of the ATM lipid kinase family. Because several members of this pathway are conserved among eukaryotes, it is likely that a RAD53-related kinase will function downstream of the human ATM gene product and play an important role in the mammalian response to DNA damage.

Original languageEnglish (US)
Pages (from-to)357-360
Number of pages4
JournalScience
Volume271
Issue number5247
StatePublished - Jan 19 1996

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Ataxia Telangiectasia
Cell Cycle Checkpoints
DNA Damage
Phosphotransferases
Yeasts
DNA Replication
Eukaryota
Transducers
Genes
Saccharomyces cerevisiae
Phosphorylation
Lipids

ASJC Scopus subject areas

  • General

Cite this

Sanchez, Y., Desany, B. A., Jones, W. J., Liu, Q., Wang, B., & Elledge, S. J. (1996). Regulation of RAD53 by the ATM-like kinases MEC1 and TEL1 in yeast cell cycle checkpoint pathways. Science, 271(5247), 357-360.

Regulation of RAD53 by the ATM-like kinases MEC1 and TEL1 in yeast cell cycle checkpoint pathways. / Sanchez, Yolanda; Desany, Brian A.; Jones, William J.; Liu, Qinghua; Wang, Bin; Elledge, Stephen J.

In: Science, Vol. 271, No. 5247, 19.01.1996, p. 357-360.

Research output: Contribution to journalArticle

Sanchez, Y, Desany, BA, Jones, WJ, Liu, Q, Wang, B & Elledge, SJ 1996, 'Regulation of RAD53 by the ATM-like kinases MEC1 and TEL1 in yeast cell cycle checkpoint pathways', Science, vol. 271, no. 5247, pp. 357-360.
Sanchez Y, Desany BA, Jones WJ, Liu Q, Wang B, Elledge SJ. Regulation of RAD53 by the ATM-like kinases MEC1 and TEL1 in yeast cell cycle checkpoint pathways. Science. 1996 Jan 19;271(5247):357-360.
Sanchez, Yolanda ; Desany, Brian A. ; Jones, William J. ; Liu, Qinghua ; Wang, Bin ; Elledge, Stephen J. / Regulation of RAD53 by the ATM-like kinases MEC1 and TEL1 in yeast cell cycle checkpoint pathways. In: Science. 1996 ; Vol. 271, No. 5247. pp. 357-360.
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