Regulation of receptor-mediated endocytosis by Rho and Rac

C. Lamaze, T. H. Chuang, L. J. Terlecky, G. M. Bokoch, S. L. Schmid

Research output: Contribution to journalArticle

315 Citations (Scopus)

Abstract

PINOCYTOSIS and membrane ruffling are among the earliest and most dramatic cellular responses to stimulation by growth factors or other mitogens. The small Ras-related G proteins Rho and Rac have a regulatory role in membrane ruffling and activated Rho has been shown to stimulate pinocytosis when microinjected into Xenopus oocytes. In contrast to these well established effects of Rho and Rac on plasma membrane morphology and bulk pinocytosis, there has been no evidence for their involvement in the regulation of receptor-mediated endocytosis in clathrin-coated pits. Here we show that activated Rho and Rac inhibit transferrin-receptor-mediated endocytosis when expressed in intact cells. Furthermore, we have reconstituted these effects in a cell-free system and established that Rho and Rac can regulate clathrin-coated vesicle formation.

Original languageEnglish (US)
Pages (from-to)177-179
Number of pages3
JournalNature
Volume382
Issue number6587
DOIs
StatePublished - 1996

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Pinocytosis
Endocytosis
Clathrin-Coated Vesicles
Clathrin
Transferrin Receptors
Membranes
Monomeric GTP-Binding Proteins
Cell-Free System
Xenopus
Mitogens
Oocytes
Intercellular Signaling Peptides and Proteins
Cell Membrane

ASJC Scopus subject areas

  • General

Cite this

Lamaze, C., Chuang, T. H., Terlecky, L. J., Bokoch, G. M., & Schmid, S. L. (1996). Regulation of receptor-mediated endocytosis by Rho and Rac. Nature, 382(6587), 177-179. https://doi.org/10.1038/382177a0

Regulation of receptor-mediated endocytosis by Rho and Rac. / Lamaze, C.; Chuang, T. H.; Terlecky, L. J.; Bokoch, G. M.; Schmid, S. L.

In: Nature, Vol. 382, No. 6587, 1996, p. 177-179.

Research output: Contribution to journalArticle

Lamaze, C, Chuang, TH, Terlecky, LJ, Bokoch, GM & Schmid, SL 1996, 'Regulation of receptor-mediated endocytosis by Rho and Rac', Nature, vol. 382, no. 6587, pp. 177-179. https://doi.org/10.1038/382177a0
Lamaze C, Chuang TH, Terlecky LJ, Bokoch GM, Schmid SL. Regulation of receptor-mediated endocytosis by Rho and Rac. Nature. 1996;382(6587):177-179. https://doi.org/10.1038/382177a0
Lamaze, C. ; Chuang, T. H. ; Terlecky, L. J. ; Bokoch, G. M. ; Schmid, S. L. / Regulation of receptor-mediated endocytosis by Rho and Rac. In: Nature. 1996 ; Vol. 382, No. 6587. pp. 177-179.
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